Amylases are one of the most important enzymes in present-day biotechnology. The objective of this study was to isolate and identify a potential thermophilic amylase producing bacterial strain from hot spring of Bakreswar village, Suri, West Bengal (India). Among the few isolated amylolytic strains on starch agar medium, the strain IB-A showed best amylase activity. Phylogenetic analysis based ...

An extracellular fl-amylase from Clostridium thermosulphurogenes was purified 811-fold to homogeneity, and its general molecular, physico-chemical and catalytic properties were determined. The native enzyme was a tetramer of 210 kDa composed of a single type subunit; its 20 amino acid N-terminus displayed 45 % homology with Bacillus polymyxa /1-amylase. The ,-amylase was enriched in both acidic...

A simple, rapid screening procedure for determining the relative amounts of pancreatic (P)- and salivary (S)-type amylase in serum is presented, involving incorporation of a selective inhibitor (from wheat-germ) into commercially available BMD Single-Vial Amylase and Beckman Enzymatic Amylase-DS procedures for manual and automated isoamylase measurements. Optimal concentrations of inhibitor inh...

The presence of ar-amylase in liver tissue has been reported previously by several investigators (l-3). In the liver from rats deprived of food, the enzyme is found primarily in the microsomal fraction (4) whereas in fed animals a considerable proportion is bound to glycogen (5). In perfused liver systems, amylase activity is secreted into the perfusion medium under conditions in which other en...

A study was carried out to assess the effect of total body irradiation on pancreatic and parotid isoenzymes of amylase in patients about to undergo bone-marrow transplantation who had received high-dose cyclophosphamide. Twelve patients were studied, enzyme activity being measured before and at various times after total body irradiation. Serum total amylase activity rose rapidly within 12 hours...

The gene encoding the hyperthermophilic extracellular alpha-amylase from Pyrococcus furiosus was cloned by activity screening in Escherichia coli. The gene encoded a single 460-residue polypeptide chain. The polypeptide contained a 26-residue signal peptide, indicating that this Pyrococcus alpha-amylase was an extracellular enzyme. Unlike the P. furiosus intracellular alpha-amylase, this extrac...

This paper presents evidence for the occurrence of proteinaceous inhibitors isolated from Chick pea (Cicer arietinum), Kidney bean (Phaseolus vulgaris), Maize seeds (Zea mays), Wheat (Triticum aestivum) and Millet (Pennisetum typhoides). These seeds inhibitors exhibited inhibitory activity against alpha amylase from red flour beetle, T. castaneum. Out of five tested seed samples Wheat, Maize an...

Remazolbrilliant Blue covalently linked to amylose (Amylose Azure) has been used for the determination of ex-amylase (Rinderknecht, Wilding, and Haverback, 1967). It forms an insoluble suspension in aqueous media, but liberates a soluble highly coloured blue dye as a result of amylase activity. This communication describes the use of this compound for the demonstration of amylase isoenzymes sep...

A semi-quantitative reverse transcription-polymerase chain reaction (RT-PCR) assay was developed to estimate tissue-specific expression of two amylase genes in five different tissues of the Pacific oyster Crassostrea gigas and the experimental effects of food level on the cellular, enzymatic and gene expression changes of the digestive gland. Results indicated that both genes were transcribed a...