Electrochemical biosensors are transducers that convert biological information into electrical information. Electrochemical biosensors provide qualitative and quantitative information (Wang 1999) on the existence and concentration of the target compounds in the analyte in the form of current (amperometric biosensor) or voltage (potentiometric biosensor). A typical amperometric biosensor consist...

An amperometric L-ascorbic acid (AA) biosensor fabricated by immobilizing ascorbate oxidase (AO) in poly(3,4-ethylenedioxythiophene) (PEDOT) and multi-walled carbon nanotubes (MWCNTs) composite films was reported for the first time. The entrapment of AO in PEDOT/MWCNTs composite films was performed during an electrochemical polymerization process. The influence of various experimental condition...

In this work, laser-based wavelength-modulated differential photothermal radiometry (WM-DPTR) is applied to develop a non-invasive in-vehicle alcohol biosensor. WM-DPTR features unprecedented ethanol-specificity and sensitivity by suppressing baseline variations through a differential measurement near the peak and baseline of the mid-infrared ethanol absorption spectrum. Biosensor signal calibr...

1. Amperometric 2. Voltametric 3. Conductometric Biosensors [1]. In amperometric biosensor, current is final output, this current is measured in terms of oxygen. Whereas in Voltametric biosensor and voltage is final output. In conductometric biosensor, thermal voltage is final output. The biosensor contains a ligand and a transducer ligand means biological element and transducer mean which conv...

A novel third-generation hydrogen peroxide (H(2)O(2)) biosensor was developed by immobilizing horseradish peroxidase (HRP) on a biocompatible attapulgite (ATP) modified glassy carbon (GC) electrode. The ATP could provide a biocompatible microenvironment for enzyme molecules, greatly amplify the coverage of HRP molecules on the electrode surface, and most importantly facilitate the direct electr...

This paper reports a first microbial biosensor for rapid and cost-effective determination of organophosphorus pesticides fenitrothion and EPN. The biosensor consisted of recombinant PNP-degrading/oxidizing bacteria Pseudomonas putida JS444 anchoring and displaying organophosphorus hydrolase (OPH) on its cell surface as biological sensing element and a dissolved oxygen electrode as the transduce...

An E. coli SOS-EGFP biosensor which expresses enhanced green fluorescent protein as a reporter protein under the control of recA gene promoter in SOS response was constructed for detection of DNA damage and evaluation of DNA damaging chemicals. The chemicals that may cause substantial DNA damage will trigger SOS response in the constructed bacterial biosensor, and then the reporter egfp gene un...

In this paper, a novel amperometric glucose biosensor was constructed by alternative self-assembly of positively charged poly(diallydimethylammonium chloride) (PDDA) and negatively charged glucose oxidase (GOx) onto a 3D Nafion network via electrostatic adsorption. The amount of Nafion in the electrode and the number of the (PDDA/GOx)(n) multilayers were optimized to develop a sensitive and sel...

A novel needle-type biosensor based on carbon nanotubes is reported. Thebiosensor was prepared by packing a mixture of multi-wall carbon nanotubes (MWCNTs),graphite powder and glucose oxidase (Gox) freeze-dried powder into a glass capillary of 0.5mm inner diameter. The resulting amperometric biosensor was characterizedelectrochemically using amperometry in the presence of hydrogen peroxide and ...

In this study, we developed a highly sensitive amperometric biosensor for glucose detection based on glucose oxidase immobilized in a novel carbon nanosphere (CNS)/sodium alginate (SA) composite matrix. This hybrid material combined the advantages of CNS and natural biopolymer SA. This composite film was characterized by scanning electron microscope, electrochemical impedance spectroscopy and U...