1. The analysis, by silica-gel chromatography, of phospholipids from a human-plasma fraction is described. The fraction was derived from whole blood which had been incubated with sodium [Me-14C]acetate for 6-5 hr. The #-lipoproteins had been removed by precipitation with dextran sulphate. 2. All the phospholipids isolated were labelled. 3. The fatty acids of the isolated phospholipids were anal...

The development of novel biotherapeutics based on peptides and proteins is often limited to extracellular targets, because these molecules are not able reach the cytosol. In recent years, several approaches were proposed overcome this limitation. A plethora cell-penetrating (CPPs) was developed for cytoplasmic delivery cell-impermeable cargo molecules. For many CPPs, multimerization or multicop...

A functional ovalbumin-dextran conjugate was prepared by dry-heated storage at 60 degrees C and 65% relative humidity for 3 weeks. The emulsifying properties of the ovalbumin-dextran conjugate were about three times higher than those of an ovalbumin-glucose conjugate. SDS-electrophoresis patterns showed that the ovalbumin-dextran conjugate obtained by dry-heating was not as polydispersed as tha...

Information was sought on the cause, effects, and control of dextran in sugar manufacture in Jamaica. Storage experiments on manually harvested, burnt, whole-stalk cane revealed that Leuconostoc mesenteroides was the predominant spoilage organism. Its growth was accompanied by significant increases in dextran content and juice viscosity. However, these parameters. did not increase linearly with...

The interaction between dextran and serum albumin, gamma globulin, and fibrinogen can be studied by an electrophoretic method, which depends on obtaining electrophoretic patterns, first of each protein, then of dextran, and finally of mixtures of each protein with dextran. The areas under the electrophoretic spikes for each protein, for dextran, and for the mixtures are measured. At pH's betwee...

As a novel target for breast cancer, interferon inducers have found its role as anti-angiogenic agents with diethylaminoethyl dextran (DEAE-Dextran) being a molecule used for centuries as a transfection agent. Our results herein offer an explanation for the emergence of DEAE-Dextran as an anti-tumor agent for TNBC with in-depth mechanistic approach as an anti-angiogenic molecule. DEAE-Dextran h...

The force-velocity relations of single glycerinated rabbit psoas muscle fibers at 5 degrees C were studied at maximum and half-maximum activation in the presence of 0 (control) and 39-145 g/liter dextran T-70. Resting fiber diameter decreased progressively to approximately 70% of the nondextran control as the dextran concentration was increased. Isometric force at full activation increased to a...

The steps leading to constitutive exocytosis are poorly understood. In Dictyostelium WASH complex mutants, exocytosis is blocked, so cells that take up fluorescent dextran from the medium retain it and remain fluorescent. Here, we establish a FACS-based method to select cells that retain fluorescent dextran, allowing identification of mutants with disrupted exocytosis. Screening a pool of rando...

Clinical strains of Aeromonas hvdrophlla and Aeromonas sobria were used to develop a mouse model for determining virulence potentials of food isolates. lntraperitoneal injection with these strains caused hemorrhaging and death of 10—12 week old male BALB/c mice within 24 h post challenge. Nonsupplemented injections of Aeromonas sp. suspensions resulted in a median LD 50 value of 4.9 X 10 cells,...