Alginate lyase mainly produces active alginate oligosaccharides (AOS) by degrading via β-elimination process. In this study, the Pseudoalteromonas sp. Alg6B lyase-encoding gene alg6B-7 from polysaccharide (PL)-7 family was successfully cloned, sequenced, expressed in Escherichia coli. Based on rational design and amino acid sequence alignment of various sources, four positive mutants were obtai...

Thermostable chitinases are useful for industrial and biotechnological applications. This paper reports the stabilization of chitinase from Serratia marcescens B4A through rational mutagenesis. Changing of Ser 390 to Ile in S. marcescens. The stabilization was enhanced through entropic stabilization by reduction of the loop length and also by increasing of the beta chain length. With this repla...

Nowadays, textile processing based on biotechnology has gained its importance in the view of stringent environmental and industrial safety conditions. The use of protease enzymes in protein fibers to improve some physical and mechanical properties is particularly interesting. In this research, wool yarns were first treated with different concentrations of protease enzymes in aqueous solution in...

tyrosinase is a multifunctional oxidase that is widely distributed in nature. it is a key enzyme in melanin biosynthesis and is involved in determining the color of mammalian skin and hair. in addition it is responsible for the undesirable enzymatic browning that occurs in plant-derived foods, limiting the shelf -life of fresh-cut products with the resultant economic loss.  in recent years ther...

l-asparaginase has lots of medical and industrial applications. ever since l-asparaginase anti-tumor activity was first demonstrated, its production using microbial systems has attracted considerable attention owing to their cost-effective and eco-friendly nature. the aim of this study is to obtain l-asparaginase producing bacteria and determining the enzyme activity. samples were picked up fro...

leptospirosis is a worldwide zoonosis caused by leptospira interrogans. this study was conducted toevaluate serologic and bacteriologic findings of leptospirosis in clinically-suspected cows. 380 sera and 33urine samples were collected from 6 industrial dairy farms in tehran suburb, from december 2004 to june2005. the prevalence of disease was determined by microscopic agglutination test (mat),...

background: today a large number of bacterial amylases are available commercially in industry. the goal of the present study was purification and biochemical characterization of an extracellular thermostable alkaline α-amylase from the novel moderately halophilic, bacillus persicus from the aran-bidgol, iran.   methods : purification of enzyme, was carried out by acetone precipitation, ultrafil...

BACKGROUND The rising concerns about the scarcity of fossil fuels, the emission of green house gasses and air pollution by incomplete combustion of fossil fuel have also resulted in an increasing focus on the use of cellulases to perform enzymatic hydrolysis of the lignocellulosic materials for the generation of bioethanol. The aim of this study was to isolate a potential thermo-solvent toleran...

Agricultural, forestry, and food industries produce large amounts of lignocellulosic wastes every year. Land disposal these residues without proper treatment leads to environmental pollution negative health effects. The recent advances in valorization agro-industrial by the production lignocellulolytic enzymes under solid-state fermentation (SSF) are reviewed. SSF is a promising technology enzy...

The aim of this paper is twofold: first, to explore the effects of industrial cluster on innovation capacity, and second, to study the impact of external resources on firms’ innovation capacity especially under Chinese regional economic policy. This is a critical subject that lacks sufficient analysis. Through years of investment and effort, several IT industrial clusters have developed in Chin...