A procedure is described for the purification of cytochrome c peroxidase from Pseudomonas aeruginosa involving extraction by sonication, followed by acid precipitation and chromatography on only two types of gel. The final preparation had a purity ratio A407/A280 of 4.2, and was found to be essentially pure by isoelectric focusing. The enzyme was shown to be unstable during degassing under vacu...

Objective: Today, special attention is paid to the use of zirconium dioxide nanoparticle (nano-ZrO2), a neutral bioceramic metal, particularly for drug and gene delivery in medicine. However, there are some reports implying that use of nano-ZrO2 is associated with cytotoxic effects like inhibiting the cell proliferation, DNA damage and apoptosis. In the present study, we examined whether nano-Z...

A light-addressable potentiometric (silicon) sensor was used in an immunofiltration procedure for the detection of pathogenic bacteria. Yersinia pestis was detected by filtering the cells onto nitrocellulose membranes and then filtering anti-Y. pestis mouse monoclonal antibody and anti-mouse immunoglobulin G-horseradish peroxidase conjugate. For Neisseria meningitidis detection, mouse monoclona...

Saccharomyces cerevisiae was obtained from palm wine and used for the extraction of peroxidase. Peroxidase isolated partially purified characterized. A twelve-day pilot study carried out which gave highest activity on day eight. The crude enzyme-specific –0.751 U/mg with 70% ammonium sulfate precipitation peroxidase achieved. precipitation, following gel filtration Sephadex G-100. Two peaks con...

The present work was undertaken to study the effects of 28-homobrassinolide on growth, zinc metal uptake, antioxidative enzyme [guaiacol peroxidase (EC 1.11.1.7), catalase (EC 1.11.1.6), glutathione reductase (EC 1.6.4.2), ascorbate peroxidase (EC 1.11.1.11), superoxide dismutase (EC 1.15.1.1)] activities and protein content in 7-d-old seedlings of Brassica juncea L. treated with Zn metal at di...

BACKGROUND The pathophysiology of migraine and other headaches is still unknown, and research is mostly done on neurotransmitter, biochemical and vascular mechanisms. The aim of this study was to examine the role of antioxidant enzymes in the pathophysiology of headache in the interictal period of the pain process. METHODS In this study, glutathione peroxidase, catalase and superoxide dismuta...

Ascorbate peroxidase, a haem protein (EC 1.11.1.11), efficiently scavenges hydrogen peroxide (H(2)O(2)) in cytosol and chloroplasts of plants. In this study, a full-length coding sequence of thylakoid-bound ascorbate peroxidase cDNA (TatAPX) was cloned from a drought tolerant wheat cultivar C306. Homology modeling of the TatAPX protein was performed by using the template crystal structure of ch...

Environmental pollution has been a serious problem since the start of the industrial revolution. The pollution usually comes from industrial wastes, waste water, heavy metal, etc. Heavy metals such as lead, mercury, tin are considered to be toxic to living organisms and enzyme activities. However, metal ions can play essential role in plants and animals as they are involved in fundamental proce...

Soil stability includes both resistance, the ability to withstand a perturbation or stress, and resilience, recover pre levels. The functional of soil microbial communities is paramount importance for ecosystem functioning. We investigated differences in (resistance resilience) three enzyme activities (hydrolytic, laccase peroxidase) different forest (holm oak, black pine beech) soils after add...