Natural transformation is assumed to be the most likely mechanism by which DNA from transgenic plants could be horizontally transferred to bacteria. In order to determine the occurrence of naturally transformable bacteria amongst bulk and rhizosphere soil bacteria, different transformation strategies were employed using either plasmid DNA (IncQ plasmids pSM1890 and pSM1885, conferring GFP, Smr,...

This study investigated bacterial growth-inhibitory effect of 69 therapeutic herbal plants extracts on 9 bacterial strains using a disc diffusion assay. Especially, the antimicrobial activity of Psoraleae semen, which showed different activity on pathogenic Gram-positive and Gram-negative bacteria, was evaluated by MIC (minimal inhibition concentration) and biofilm formation assay. The effect o...

Natural aquatic and soil samples were screened for the presence of thiocyanate-degrading bacteria. Using thiocyanate supplementation, we established an enrichment culture containing such bacteria from lake water. The dominant bacteria had the scnC-LS5 gene encoding thiocyanate hydrolase, which was closely related to the enzyme found previously in Thiobacillus thioparus THI115 isolated from acti...

use of herbal extracts and essences as natural antibacterial compounds has become increasingly popular for the control of oral infectious diseases. therefore, finding natural antimicrobial products with the lowest side effects seems necessary. the present study sought to assess the effect of punica granatum l. water extract on five oral bacteria and bacterial biofilm formation on orthodontic wi...

Among cultivated and characterised ammonia oxidising archaea (AOA), representatives of Candidatus Nitrosocosmicus are unique in their ability to grow at high concentration (up 100 mM), concentrations that tolerated by many bacteria (AOB). These strains also a wide range incubation temperatures (4–45 °C), with highest oxidation rates relatively temperature (28–40 °C). In addition, oxidiser growt...

licorice (glycyrrhiza glabra l.) is an important medicinal plant. in this study, the antimicrobial activities of ethanolic and aqueous extracts from licorice leaves were studied compared to root extracts activities. bacillus subtilis, enterococcus faecalis, klebsiella pneumoniae, pseudomonas aeruginosa, staphylococcus aureus and escherichia coli, and candida albicans were used as test organisms...

The large-scale release of wild-type or genetically modified bacteria into the environment for control of plant diseases or for bioremediation entails the potential risk of groundwater contamination by these microorganisms. For a model study on patterns of vertical transport of bacteria under field conditions, the biocontrol strain Pseudomonas fluorescens CHA0, marked with a spontaneous resista...