نتایج جستجو برای: protein assay

تعداد نتایج: 1391263  

Journal: :Organic agriculture 2022

Abstract The knowledge of the precaecal digestibility crude protein (CP) and amino acids (AA) has gained importance. However, since animal welfare is given high priority, experiments are recommended to be reduced or replaced. Thus, in vitro approaches interest for feed evaluation. present study aimed an adaption a multi-enzyme assay. An assay predict CP AA feedstuffs swine was adapted standardi...

ابراهیمی, فیروز , جوادی, غلامرضا, مداح, بزرگمهر , پورانوری, سارا ,

Background and purpose: Human epidermal growth factor (hEGF) is a polypeptide of 53 amino acids with various medical application such as wound healing. The purpose of this study was cloning, expression, and purification of recombinant human EGF (rhEGF) and assessment of its mitogenic effect on NIH 3T3 cells. Materials and methods: Subcloninig of hEGF was performed in to pET24a (+). Protein e...

Journal: :Agronomy 2023

Protein content is one of the main factors determining end-use quality wheat. NO APICAL MERISTEM-B1 (NAM-B1) a major gene regulating wheat grain protein content. The present study aimed to identify new genetic resources using wild-type NAM-B1 allele breed high-protein-content cultivars. We genotyped HIGH GRAIN PROTEIN CONTENT-B1 (GPC-B1) locus and in 165 A kompetitive allele-specific polymerase...

Journal: :Processes 2023

It has been estimated and demonstrated that the antioxidant capacity of proteins is increased as a result digestion in gastrointestinal tract, which can be contributed by denaturation digestion. This study aimed to evaluate effect proteolytic on activity bovine serum albumin (BSA) chicken egg white model systems. Denaturation with an anionic detergent (sodium dodecyl sulfate) papain trypsin act...

Journal: :Neurology 2021

A 58-year-old man developed subacute gait instability, oscillopsia, and vertigo. Examination showed ataxia periodic alternating nystagmus (PAN) (video). MRI revealed T2 hyperintensities in the left cerebellum nodulus (figure). CSF was inflammatory. Autoantibody evaluation positive for Kelch-like protein 11 (KLHL11) serum by tissue-based immunofluorescence assay cell-based assay, prompting ident...

Journal: :Clinical science and molecular medicine 1974
S Edelstein M Charman D E Lawson E Kodicek

1. A competitive protein-binding assay for plasma 25-hydroxycholecalciferol was developed, using Sephadex LH-20 columns for the isolation of the sterol and a partially purified protein from rat serum as the binding protein. 2. The mean plasma 25-hydroxycholecalciferol in eighteen male and female normal volunteers was 38 5 SD 14 pmol/ml (1 5.2 & 5.6 nglml). 3. Levels of 25-hydroxycholecalciferol...

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