New bioactive proteins need to be screened from various microorganisms for the increasing need for industrial and pharmaceutical peptide, proteins, or enzymes. A novel polymerase chain reaction (PCR) method, restriction site-dependent PCR (RSD-PCR), was designed for rapid new genes cloning from genomic DNA. RSD-PCR strategy is based on these principles: (i) restriction sites disperse throughout...

msatcommander is a platform-independent program designed to search for microsatellite arrays, design primers, and tag primers using an automated routine. msatcommander accepts as input DNA sequence data in single-sequence or concatenated, fasta-formatted files. Search data and locus-specific primers are written to comma-separated value files for subsequent use in spreadsheet or database program...

AIM To assess the association between ABO blood group genotypes and genetic risk factors for thrombosis (FV Leiden, prothrombin G20210A, and methylenetetrahydrofolate reductase C677T mutations) in the Croatian population and to determine whether genetic predisposition to thrombotic risk is higher in non-OO blood group genotypes than in OO blood group genotypes. METHODS The study included 154 ...

background: regulatory cd4+t (treg) cells are effective in maintaining immune tolerance. objective: to investigate single nucleotide polymorphisms (snps) of transforming growth factor β-1 (tgf-β1) and forkhead box protein 3 (foxp3) genes in iranian patients with allergic rhinitis (ar). methods: variations at codons 10 and 25 of tgf-β1 and foxp3 at positions -3279 a>c and -924 a>g were evaluated...

RT-PCR assays for detection of BRSV, based on four different sets of primers were optimized and evaluated for their sensitivity and specificity. Primers used in this study were specific for genes encoding three BRSV proteins, nucleoprotein N and glycoproteins F and G. Our results indicated that RT-PCR with primers B7:B8 for G protein was the most efficient in detecting BRSV. Starters B7:B8 reac...

The Yellow Mosaic Disease is the main constraint for decreased yield in blackgram production. Coat protein gene of Mungbean yellow mosaic virus (MYMV) has been characterized and phylogenetic tree was constructed. genomic DNA from diseased plants collected it tested it’s quality quantity using a Nanodrop. isolated subjected to polymerase chain reaction begomovirus universal primers preliminary c...

Objective(s): Juvenile idiopathic arthritis (JIA) is one of the most common chronic rheumatic diseases in children. The complex nature of this immune-mediated disease owes itself to several predisposing genes and environmental factors affecting its pathogenesis. Conducted in Iran, this study was originally intended to investigate every possible association between HLA DRB1 alleles and a suscept...