Three methods of determining lipase activity were optimized, validated, and compared using skim and whole milk. A chromogenic ester (p-nitrophenyl caprylate) was used in all to quantify the enzyme activity through the release of p-nitrophenol. It was measured colorimetrically (method A) or spectrophotometrically (methods B and C) with a clarifying reagent to render the samples measurable. Metho...

Cottage cheese was produced from reconstituted skimmed milk powder Fer­ mentation of skim milk with approximately 11.16% total solids was performed at 22 °C with 0,5% mesophilic starter culture, "O" type (DVS, Chr Hansen's Lab), without rennet addition. Dry Cottage cheese samples contained an average 21,32% total solids; 17.85%) proteins; 0.75%) ash; and 90.33 mg Ca/lOOg. The Cottage cheese pro...

BACKGROUND A positive correlation between an individual's cholesterol level and development of CHD has been suggested. Low levels of high-density lipoprotein cholesterol (HDL-C) and high levels of low-density lipoprotein cholesterol (LDL-C) are important risk factors and oxidation of LDL has been implicated as an initiator of atherosclerosis. AIM Attempts are being made worldwide for the sear...

The activity of the lipase(s) of two strains of coagulase-positive Staphylococcus aureus was determined in milk fat incubated at 15, 22, and 30 C for 8 days. Total fat hydrolysis was measured by acid degree values (ADV). Neutral lipids were separated into component groups on a Florisil column. Free fatty acids were determined by temperature-programmed gas-liquid chromatography. The ADV were 25 ...

Fluidized bed agglomeration is used to produce large and porous dry agglomerates with improved instant properties. Water (or binder solution) is sprayed in the fluidized bed of particles to render their surface sticky. The agglomerate growth results from the repetition of different steps (wetting of the particle surface, particles collision and bridging, and drying) and depends on the processin...

The physical and chemical changes of 4x milk concentrates prepared by ultrafiltration (UF) microfiltration (MF) combined with diafiltration were studied after heating in situ to 85 °C subsequent cooling. Skim (1x) was analyzed as control. had comparable pH, colloidal casein calcium phosphate, but differed whey proteins serum allowing investigating the effect soluble phase composition on viscosi...