Among the different types of bacteria being exploited as live vaccines Bacillus endospores have a number of distinct advantagesmost importantly being their heat stability. Typically antigens are displayed on the spore surface or expressed in the germinating spore, i.e., the vegetative cell. Howantigens are delivered by spores can significantly affect the nature of the resulting immune response....

The microsporidian spore extrusion apparatus activates with a calcium influx from Spraguea lophii spore wall/plasma membrane; this influx requires preconditioning with an extrasporular shift in medium pH to the alkaline in the presence of the polyanions mucin or polyglutamate. Undischarged S. lophii spores display calcium bound to the wall/plasma membrane with a characteristic calcium-chlorotet...

Many protozoa form spores in response to adversity; therefore, spore germination is a key process in their life cycle. Dictyostelium discoideum sporulates in response to starvation following a developmental program. Germination is characterized by two visible changes, spore swelling and the emergence of amoeba from the spore capsule. Several studies have indicated that an additional process ter...

An infection of pearl oysters, Pinctada maxima, attributed to a Haplosporidium sp. by Hine and Thorne (1998) has been detected on 3 occasions and is considered to represent a serious concern to the pearling industry in Australia. The spore ornamentation of the parasite was determined by scanning electron microscopy and transmission electron microscopy. Spores of the parasite were pleomorphic, o...

The combination of genomic and proteomic analyses is a useful tool for the study novel Bacillus thuringiensis (Bt) strains, as these approaches allow accurate identification pesticidal proteins virulence factors produced. Here, we isolated evaluated potential Neotropical Bt strain (TOD651) controlling larvae Aedes aegypti Culex quinquefasciatus mosquitoes. Aiming full comprehension TOD651 larvi...

The objective of this study was to formulate and evaluate the drug-polymer interaction of mefenamic acid (MA) using two polymers with different characteristics as ethylcellulose (EC) and/or cellulose acetate phthalate (CAP). Microspheres were prepared by the modified emulsion solvent evaporation (MESE). The effect of drug-polymer interaction was studied for each of microspheres. Important param...

Designed ankyrin repeat proteins (DARPins) that specifically bind to almost any target can be obtained by ribosome display or phage display from combinatorial libraries. Although DARPins are already very stable molecules, molecular dynamics simulations, equilibrium denaturation experiments, structural studies, and recent NMR experiments suggested that the unfolding of the original C-terminal ca...

biological control of tomato root-knot nematode, meloidogyne javanica, by the isolate bi of trichoderma harzianum was evaluated in greenhouse and laboratory conditions in tomato cv. king stone. effects of fungal spore suspensions in various densities (103 to 108 spore/ml) were studied on different disease intensities (average of gall diameters, average of root weight and average of stem weight)...