In vitro culture systems using bone marrow cells from BALB/C mice were set up in medium supplemented with spleen-derived medium. Bone marrow cells grown in spleen-derived medium gave rise to cultures containing >97% mast cells. The cells were used in polarisation chemotaxis assays with the intention of determining the effect of nerve tissue-derived neurotransmitters on mast cell migration. ...

abstract somatic embryogenesis is a new approach to in vitro plant propagation. this technique is based on stem cell obtain and its differentiation to a complete plant. this is affected by components of media culture. so, the effects of ms, b5 and nl media on carrot petiole somatic embryogenesis investigated via histological experiment. to do this, carrot petioles (with 1 cm dimension) cultured...

Some dogs display behaviors such as excessive barking and jumping. Unwanted behaviors can often lead to animal abuse or pet relinquishment to a shelter. This study focused on the effects of androstenone on the behavior of dogs showing a barking and jumping syndrome. In the first study, barking dogs were sprayed with (a) nothing, (b) a placebo spray with sound or noise, (c) the same as (b) plus ...

Two strains of callus have been isolated from bean hypocotyl and grown on a defined maintenance medium supplemented with 2 mg/l. 2:4-dichlorophenoxyacetic acid (2:4D) and 2% sucrose. Root initiation was observed in one strain and formation of nodules containing xylem and phloem in both strains after transfer to an induction medium supplemented with 1 mg/l. naphthyleneacetic acid, 0-2 mg/l. kine...

The aim of this study was to isolate mouse embryonic stem cells from late blastocyst stage embryos and to use them as a model system for the study of hematopoietic induction outside the embryo by coculturing of embryonic stem cells with bone marrow stromal cells. Blastocyst stage embryos from pregnant NMRI mice were obtained and cultured for 1-2 days in DMEM medium. The inner cell masses formed...

salicylic acid play an important role in plants physiological processes. in this research the effect of salicylic acid on induction of somatic embryogenesis and plant pigments synthesis in the phloem and petiole explants of carrot in the b5 medium was investigated. we added treatments with 0, 0.5, 1.5, 2 and 3 mm to culture medium by experimental base on the randomized design to investigate the...

INTRODUCTION This work shows that 3% (v/v) human urine (HU) in semisolid Liver Infusion Tryptose (SSL) medium favors the growth of Trypanosoma cruzi and T. rangeli. METHODS Parasites were plated as individual or mixed strains on SSL medium and on SSL medium with 3% human urine (SSL-HU). Isolate DNA was analyzed using polymerase chain reaction (PCR) and pulsed-field gel electrophoresis (PFGE)....

in order to investigation of vermicompost effect on growth and yield of marigold in pot medium, an experiment was done by a randomized completely block design in three raplications. treatmnets were included control (30% v/v of soil plus 70% v/v of sand) and three levels of vermicompost (20, 40, 60 % v/v of vermicompost+30% v/v of sand and soil) that applied in three lit. pots. marigold seeds (t...