Bretaudiere J. Factor VII gene intronic mutation in a lethal Factor VII deficiency: effects on splice-site selection. Blood 2003; 102: 561–3. 10 Rosen E, Chan JCY, Idusogie E, Clotman F, Vlasuk G, Luther T, Jalbert LR, Albrecht S, Zhong L, Lissens A, Schoonjans L, Collen D, Costellino FJ, Carmeliet P. Mice lacking Factor VII develop normally but suffer fatal perinatal bleeding. Nature 1997; 390...

Mucopolysaccharidosis VII (MPS VII) is a lysosomal storage disorder characterized by a deficiency in beta-glucuronidase activity, leading to systemic accumulation of poorly degraded glycosaminoglycans (GAG). Along with other morbidities, MPS VII is associated with pediatric spinal deformity. The objective of this study was to examine potential associations between abnormal lumbar spine matrix s...

Human coagulation Factors VII and VIIa bind with equal affinity to monocytes stimulated with endotoxin. Equilibrium binding studies performed at 0 degrees C using 125I-labeled Factor VII and VIIa showed the dissociation constant (Kd) to be congruent to 82 pM with congruent to 3,600 binding sites/monocyte. Ca++ was required for Factor VII and VIIa interaction with monocytes (optimal CaC12 concen...

Hematol Transfus Int J 2017, 4(2): 00078 those using the anti-coagulant agent, warfarin [4,5]. One case of a patient undergoing coronary artery bypass grafting was noted among 7cases of surgery-associated acquired hemophilia [2]. A 66-year-old man underwent aortic valve replacement for severe aortic valve stenosis. After surgery, he was administered warfarin (2mg/day) for anticoagulation and th...

Subcellular membrane and granule fractions derived from human platelets contain factor VIIII antigen and von Willebrand factor activity but not factor VII procoagulant activity. Circulating platelets constitute a significant reservoir of plasma factor VIII antigen, containing approximately 15% of the amount of factor VIII antigen present in platelet-poor plasma. The antibiotic ristocetin, which...

Previous results, presented in abstract form, indicate that replacement of thromboplastin with a mixture of phospholipid and truncated soluble tissue factor apoprotein results in a coagulation assay that can directly measure plasma factor VIIa levels without interference from zymogen factor VII (Atherosclerosis Thromb 11:1544a, 1991 [abstr]). We have exploited the specificity and sensitivity of...