A validated HPLC method was developed to determine doxorubicin concentration in a small volume of rat plasma (60 µl) with convenient fluorescence detection. Sample preparation includes a simple one-step liquid-liquid extraction using minimum amount of organic solvent, with extraction recoveries more than 95%. The analysis was accomplished using PerfectSil C18 column maintained at 35 °C and a mo...

A rapid and sensitive HPLC method has been developed for the quantification of mirtazapine (MRZ), a noradrenergic and specific serotonergic inhibitor antidepressant (NaSSA) and its two major metabolites N-desmethylmirtazapine (NDM) and 8-hydroxymirtazapine (8-OHM) in human plasma. The separation was achieved using Chromolith C18 column and a mobile phase of acetonitrile: phosphate buffer (pH=3,...

Plasma DNA has been measured by a new physiochemical approach based on fluorimetric analysis of phenol extracted nucleic acids electrophoresed on polyacrylamide gels. This method is specific for native double-stranded DNA of molecular weight greater than 5 x 10(6). The use of plasma rigorously freed of leucocytes is essential to prevent falsely high values. When such precautions are taken level...

In order to meet the design requirements of lightweight artillery and adopt method double-glow plasma nitriding solve problem low hardness poor abrasion resistance Ti alloy, BTi-62421S high-performance titanium (Ti) alloy was selected as experimental material replace gun steel. To study effect different nitrogen (N) concentrations on heat scouring performance investigate influence alloying elem...

results mean ± standard deviation (sd) of plasma zinc levels was determined as 0.78 ± 0.22 mg/l. also zinc level was 0.76 ± 0.19 mg/l and 0.80 ± 0.24 mg/l in thalassemic and non thalassemic patients, respectively. t-test analysis showed that there is no significant difference between these two groups regarding plasma zinc level (p = 0.235). conclusions it is concluded that zinc level of studied...

With technology scaling, vulnerability to soft errors in random logic is increasing. There is a need for on-line error detection and protection for logic gates even at sea level. The error checker is the key element for an on-line detection mechanism. We compare three different checkers for error detection from the point of view of area, power and false error detection rates. We find that the D...

Phenotype and/or genotype misclassification can: significantly increase type II error probabilities for genetic case/control association, causing decrease in statistical power; and produce inaccurate estimates of population frequency parameters. We present a method, the likelihood ratio test allowing for errors (LRTae) that incorporates double-sample information for phenotypes and/or genotypes ...

Plasma lensing is the refraction of low-frequency electromagnetic rays due to cold free electrons in universe. For sources at a cosmological distance, there observational evidence elongated, complex plasma structures along line sight requiring multi-lens-plane description. To investigate limits single-plane lensing, we set up double-plane lens with projected Gaussian electron density each plane...

background: a simple and sensitive high performance liquid chromatography-electrospray ionization mass spectrometry method has been evaluated for the assignment of clonidine hydrochloride in human plasma. methods: the mobile phase composed of acetonitrile-water 60:40 (v/v) and 0.2% formic acid 20 µl of sample was chromatographically analyzed using a repacked zorbax-xdb-ods c18 column (2.1 mmx30...