Intimate swabs taken for examination in sexual assault cases typically yield mixtures of sperm and epithelial cell types. While powerful, differential extraction protocols to overcome such type by separate lysis cells spermatozoa can still prove ineffective, particular if only few are present or contain from more than one individual leading complex low level DNA mixtures. A means avoid consists...

Although an inverse relationship is expected in ancient DNA samples between the number of surviving DNA fragments and their length, ancient DNA sequencing libraries are strikingly deficient in molecules shorter than 40 bp. We find that a loss of short molecules can occur during DNA extraction and present an improved silica-based extraction protocol that enables their efficient retrieval. In com...

Recent developments in sequencing methods have enabled whole genome sequencing of several species and the available sequence information has allowed the development of high throughput genotyping chips. However, these genotyping methods require high quality DNA. The possibility to genotype samples based on DNA from non-invasive sources would permit retrospective genotyping of previously collecte...

background: colorectal cancer, the third most common type of cancer is a major cause of mortality worldwide. if colorectal cancer is detected at the early stages, the 5-year survival rate is 90%. meis1 homeobox gene is implicated in numerous solid tumors and hematological malignancies. therefore, the present study aims to investigate the methylation status of the meis1 gene in colorectal cancer...

BACKGROUND Obtaining chloroplast genome sequences is important to increase the knowledge about the fundamental biology of plastids, to understand evolutionary and ecological processes in the evolution of plants, to develop biotechnological applications (e.g. plastid engineering) and to improve the efficiency of breeding schemes. Extraction of pure chloroplast DNA is required for efficient seque...

we describe a rapid, simple, and reliable procedure for routine isolation of mycobacterium leprae dna from slit-skin swab specimens. this one-step dna extraction method is based on the utilization of chelexr 100, a chelating ion exchange resin. slit-skin swab specimens from seven untreated leprosy patients at baba baghi leprosy hospital in iran were processed by this procedure. the polymerase c...

Although the first ancient DNA molecules were extracted more than three decades ago, nuclear genomes could only be characterized after high-throughput sequencing was invented. Genome-scale data have now been gathered from thousands of archaeological specimens, and number biological tissues amenable to genome is growing steadily. Ancient fragments are typically ultrashort carry extensive amounts...

The recent development of homologous recombination-mediated gene targeting (GT) techniques has made it easy to modify nucleotide sequences in plant genomes. Backcross breeding following GT provides transgene-free lines with high probability. However, owing the possibility unintentional transgene introduction during genetic transformation, analytical methods may be necessary reliably and compreh...

The red palm weevil, Rhynchophorus ferrugineus , is the most harmful species among those pests affecting trees. Its impact causes important economic losses around World. Nevertheless, genetic information of Rh. very scarce. Last year, first genome assembly was published including a rough description its repeatome. However, no has been added about one main components repeated DNA, satellite DNA....