Adventitious roots, generated using leaf explants of P. nodiflora, and meristem explants of L. reticulata, were cultured on Murashige and Skoog (MS) medium supplemented with napthylacetic acid (2 microM) and indole butyric acid (3 microM) respectively. After 30 days, subculturing of roots in liquid MS medium with napthylacetic acid (1.5 microM) for P. nodiflora and indole butyric acid (3 microM...

the jatropha curcas l., a plant belongs to euphorbiaceae family, which has great economic and medicinally value. there is high amount of oil in seeds of this plant. because of low germination ability of seed, propagation of jatropha curcas via seed is not usual. the purpose of this work is optimizing of jatropha curcas tissue culture for optimization of callus formation and propagation through ...

Hairy root culture is an effective method to produce secondary metabolites. In fact, hairy roots are genetically and biologically stable and also they are able to produce metabolites without hormones. Chicory (Cichorium intybus L.) is one of the most important medicinal plants with valuable medicinal compounds. In this research, hairy roots were induced by different Agrobacterium rhizogenes str...

In rubber, the current propagation method of grafting on to unselected seedlings, maintains intraclonal heterogeneity for vigour and productivity and hence a great improvement may be expected by using micropropagation in vitro. Micropropagation with nodal and shoot tip explants derived from seedlings are possible as with mature clonal explants. The major problem in using clonal material from ma...

Ten-day rat embryos have been cultivated in vitro, with different layers of the extraembryonic membranes removed. The effects of presence or absence of each membrane on the morphology of the embryos, their histodifferentiation and their uptake of leucine into protein have been followed. Explants with all membranes left intact failed to expand fully and to undergo axial rotation of the embryo, b...

Persian shallot, also called leek (Allium ampeloprasum), is a monocotyledon plant of the lily family (Liliaceae). It belongs to the genus Allium, has a characteristic taste and morphological features, and is considered as one of the oniony vegetables. This research was conducted with the purpose of obtaining optimal conditions for tissue culture of Persian shallot and of comparing calli and lea...

Turtle (Emys orbicularis L.) embryo thymuses grafted in the somatopleure or onto the quail embryo chorioallantoic membrane developed in these heterotopic sites for 2-12 days. When the thymus was removed from embryos at early stages such that no thymocytes were yet present during normal development, epithelial cells with mitoses were observed in the explants but no turtle thymocytes developed wh...