Analysis of the purity and concentration DNA isolation results was carried out using samples dragonflies (Onychogomphus forcipatus) species insects. This research is important because there are many kinds in molecular field that require good techniques to support success research. The purpose this paper provide information about quality isolated seen parameters. extraction method uses centrifug...

Plant tissues contain abundant polysaccharides, phenolic compounds and other metabolites, which makes it difficult to isolate high-quality RNA from them. In addition, Neolamarckia cadamba contains large quantities of other components, particularly RNA-binding alkaloids, which makes the isolation even more challenging. Here, we describe a concise and efficient RNA isolation method that combines ...

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Arils from six pomegranate (Punica granatum L.) varieties grown in the Mediterranean region of Turkey were tested for their antimicrobial properties by the agar diffusion and minimum inhibitory concentration (MIC) methods against seven bacteria: (Bacillus megaterium DSM 32, Pseudomonas aeruginosa DSM 9027, Staphylococcus aureus Cowan 1, Corynebacterium xerosis UC 9165, Escherichia coli DM, Ente...

RNA isolation is performed everyday by molecular biologists for a wide range of applications. No matter what protocol is used and how meticulous the user is, a risk to degrade RNA during the process or to carry over contaminants always exists which may affect the reliability of the subsequent analytical techniques. The parameters that are usually checked for quality control include the concentr...

Background: The prevailing methods for the genomic DNA extraction of Sclerotium rolfsii from its mycelium mat is often time consuming and yields poor quality quantity owing to presence outrageous magnitude mucilage polysaccharides. Methods: A new fast track method isolation resting structure (sclerotia) S. by modifying CTAB deprived supplementation proteinase K has been standardized. Result: pr...

Drought stress is the most important factor limiting successful production of agricultural crops worldwide. The response some almond cultivars grafted on GF677 rootstock was studied to drought at a research greenhouse in Horticultural Science Research Institute Karaj, Iran, 2016-2017 growing season. plant materials included 12 (Prunus dulcis Mill.) genotypes/cultivars, including ‘Sh10’, ‘Saba’,...

Soil analysis is crucial for estimating the risk of crop damage by root-knot nematode Meloidogyne hapla. Here, we developed an assay based on Loop-mediated Isothermal Amplification (LAMP). The LAMP primers were verified specificity against 10 different species. A manual soil DNA extraction, referred to as SKMM, was and compared with a FastDNA kit followed purification. extracted both methods fr...