Marker assisted selection (MAS) is a tool for breeding, screening, and genetic characterization of germplasm. Allelic variation of both high and low molecular weight glutenin subunits (HMW/LMW-GS) is associated with the rheological properties of wheat flour. In this study, we investigated glutenin pattern using SDS-PAGE and their PCR based on DNA markers in 60 advanced wheat lines and cultivars...

The allelic variation at seven prolamin loci involved in quality has been studied in a set of durum wheat landraces from all the Spanish regions where this crop has been traditionally cultivated. The genetic variability was higher than that found in other germplasm collections. All the loci, except Glu-B2, displayed a genetic variability higher than 0.62, with Glu-3 the most polymorphic. In tot...

The B low Mr subunits of glutenin of the F2 generation from three durum wheat crosses were analysed. Three new alleles were found at three different loci: Glu-A3i coding for 5C20 subunits, Glu-B2c coding for subunit 12* and Glu-B3l coding for 1C3C13*C16 subunits. The genetic distances between Glu-A3-Gli-A1, Glu-B2-Gli-B1, Glu-B3-Glu-B2 and Glu-B3-Gli-B1 were calculated. The effects of the allel...

The linkage relationships in wild emmer wheat, Triticum dicoccoides , between nine enzymatic loci (Mdh-1, Ipo, beta-Glu, Pept-1, Pept-3, Est-5, Est-1, 6Pgdh-2 and Hk) and a coleoptile pigment locus (Rc) were investigated. Chromosome locations of genes were inferred from analysis of ditelocentric lines of Triticum aestivum, cultivar Chinese Spring. The loci Mdh-B1 and Hk are linked (lambda = 0.1...

High molecular weight glutenin subunit composition and variation in 95 Elite-1 synthetic hexaploid (SH) wheats (Triticum turgidum/Aegilops tauschii; 2n = 6× = 42; AABBDD) were determined by sodium dodecyl sulphate polyacrylamide gel electrophoresis method (SDS-PAGE). Twenty two different alleles at Glu-1 loci in SHs were observed. Forty four different patterns of HMW-GS in synthetics were found...