نتایج جستجو برای: bacmid shuttle vector

تعداد نتایج: 203458  

Background: The importance of viral protein-2 (VP2) of canine parvovirus (CPV) in binding to human cancer cells, production of veterinary vaccines and diagnostic kits has motivated several researches on producing this protein.Objectives: Our purpose was to construct recombinant bacmid shuttle vectors expressing VP2 of CPV using Bac-to-Bac baculoviral expr...

Journal: :Jundishapur journal of microbiology 2015
Mehdi Rahpeyma Fatemeh Fotouhi Manouchehr Makvandi Ata Ghadiri Alireza Samarbaf-Zadeh

BACKGROUND Crimean-Congo hemorrhagic fever virus (CCHFV) is a member of the nairovirus, a genus in the Bunyaviridae family, which causes a life threatening disease in human. Currently, there is no vaccine against CCHFV and detailed structural analysis of CCHFV proteins remains undefined. The CCHFV M RNA segment encodes two viral surface glycoproteins known as Gn and Gc. Viral glycoproteins can ...

2014
Nima Mirzaei Talat Mokhtari Azad Rakhshandeh Nategh Hoorieh Soleimanjahi Nour Amirmozafari

BACKGROUND Sequence variations in glycoproteins of influenza virus surface impel us to design new candidate vaccines yearly. Ectodomain of influenza M2 protein is a surface and highly conserved protein. M2e in influenza vaccines may eliminate the need for changing vaccine formulation every year. OBJECTIVES In this study, a recombinant baculovirus containing M2e and cholera toxin subunit B fus...

Journal: :iranian red crescent medical journal 0
nima mirzaei department of biology, science and research branch, islamic azad university, tehran, ir iran; department of biology, science and research branch, islamic azad university, tehran, ir iran. tel: +98-2188962343 talat mokhtari azad department of virology, tehran university of medical sciences, tehran, ir iran rakhshandeh nategh department of virology, tehran university of medical sciences, tehran, ir iran hoorieh soleimanjahi department of virology, tarbiat modares university, tehran, ir iran nour amirmozafari department of microbiology, tehran university of medical sciences, tehran, ir iran

background: sequence variations in glycoproteins of influenza virus surface impel us to design new candidate vaccines yearly. ectodomain of influenza m2 protein is a surface and highly conserved protein. m2e in influenza vaccines may eliminate the need for changing vaccine formulation every year. objectives: in this study, a recombinant baculovirus containing m2e and cholera toxin subunit b fus...

Ali Jahanian-Najafabadi, Delavar Shahbazzadeh, Kamran Pooshang-Bagheri, Mahdi Behdani, Mahdi Habibi-Anbouhi, Narjes Shokrollahi,

Background: Baculovirus expression system is one of the most attractive and powerful eukaryotic expression systems for the production of recombinant proteins. The presence of a biomarker is required to monitor transfection efficiency or protein expression levels in insect cells. Methods: The aim of this study was to construct a baculovirus expression vector encoding a copepod super green fluore...

Journal: :jundishapur journal of microbiology 0
mehdi rahpeyma health research institute, infectious and tropical diseases research center, ahvaz jundishapur university of medical sciences, ahvaz, iran fatemeh fotouhi influenza research laboratory, department of virology, pasteur institute of iran, tehran, ir iran manouchehr makvandi department of virology, faculty of medicine, ahvaz jundishapur university of medical sciences, ahvaz, ir iran ata ghadiri cellular and molecular research center, faculty of medicine, ahvaz jundishapur university of medical sciences, ahvaz, ir iran alireza samarbaf-zadeh health research institute, infectious and tropical diseases research center, ahvaz jundishapur university of medical sciences, ahvaz, iran; health research institute, infectious and tropical diseases research center, ahvaz jundishapur university of medical sciences, ahvaz, iran. tel/fax: +98-6113738313

results primary, secondary, and 3d structure of cchfv gn were obtained and pcr reaction with m13 forward and reverse primers confirmed the generation of recombinant bacmid dna harboring gn coding region under polyhedron promoter. conclusions characterization of the detailed structure of cchfv gn by bioinformatics software provides the basis for development of new experiments and construction of...

2011
S Najafi F Behzadian F Fotouhi JF Mehrabadi S Hossain Zadeh

Non-replicating virus-like particles (VLPs) have been suggested as a promising platform for viral vaccines. Recently several studies have introduced VLPs produced in insect cells as a vaccine candidate against influenza infection. Neuraminidase (NA), one of the two membrane glycoproteins of influenza A viruses has been employed to construct such influenza VLPs to induce protective immunity. In ...

Journal: :iranian biomedical journal 0
narjes shokrollahi delavar shahbazzadeh kamran pooshang-bagheri mahdi habibi-anbouhi ali jahanian-najafabadi mahdi behdani

background: baculovirus expression system is one of the most attractive and powerful eukaryotic expression systems for the production of recombinant proteins. the presence of a biomarker is required to monitor transfection efficiency or protein expression levels in insect cells. methods: the aim of this study was to construct a baculovirus expression vector encoding a copepod super green fluore...

Journal: :Journal of virological methods 2007
Masato Hiyoshi Ayano Kageshima Tatsuya Kato Enoch Y Park

The bacmid system of BmMNPV with cysteine protease gene deletion (CPD-BmMNPV bacmid) was constructed using the lambda recombination system. The protease activities of Bombyx mori cells and silkworm larvae infected with this CPD-BmMNPV bacmid were reduced by 94% and 85%, respectively. By using this system, a GFP(uv)-beta1,3-N-acetylglucosaminyltransferase 2 (GFP(uv)-beta3GnT2) fusion protein was...

Journal: :The Journal of general virology 2003
Gorben P Pijlman Jessica E van Schijndel Just M Vlak

Repeated baculovirus infections in cultured insect cells lead to the generation of defective interfering viruses (DIs), which accumulate at the expense of the intact helper virus and compromise heterologous protein expression. In particular, Autographa californica multicapsid nucleopolyhedovirus (AcMNPV) DIs are enriched in an origin of viral DNA replication (ori) not associated with the homolo...

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