Immobilization is the method of entrapping/attaching the microbial or plant cells in a suitable matrix. Different methods such as encapsulation, gel entrapment, covalent bonding, cross linking and adsorption is carried out to prepare immobilized cells. When compared to enzyme immobilization, cell immobilization is regarded as a convenient method because of its low cost and improved stability. T...

This paper is concerned with the two new boundary-type radial basis function collocation schemes, boundary knot method (BKM) and boundary particle method (BPM). The BKM is developed based on the dual reciprocity theorem, while the BKM employs the multiple reciprocity technique. Unlike the method of fundamental solution, the two methods use the non-singular general solution instead of singular f...

entrapment of permeabilized whole cells within a matrix is a common method for immobilization. chitosan possesses distinct chemical and biological properties, which make it a suitable matrix for entrapment and immobilization of penicillin g acylase (pga). in the first step, escherichia coli (atcc 11105) cells were permeabilized using n-cetyl-n,n,n-trimethyl ammonium bromide (ctab) (0.1% w/v, 45...

Disc immuno-immobilization is a simple method for typing the flagellar phase of Salmonella enterica. We re-examined this method using commercial antisera, which contains the preservative sodium azide. Originally prepared motility agar activates bacterial motility and renders S. enterica resistant to sodium azide, resulting in the formation of immuno-immobilization lines around reactive immuno-d...

objective(s): enzyme immobilization via nanoparticles is perfectly compatible against the other chemical or biological approximate to improve enzyme functions and stability. in this study lactoperoxidase was immobilized onto silica-coated magnetite nanoparticles to improve enzyme properties in the presence of cadmium chloride as an inhibitor. materials and methods:  the process consists of the ...

bioelectronics has attracted increasing interest in recent years because of their applications in various disciplines, such as biomedical. development of efficient bio-nano hybrid materials is a new move towards revolution of nano-bioelectronics. a novel nano-bio hybrid electrode based on zno–protein for bioelectronics applications was prepared and characterized. the electrode was made by coval...

Article history: Received 14 January 2011 Received in revised form 31 May 2011 Accepted 8 July 2011 Available online xxxx