plant samples were collected from yazd, isfahan, fars and tehran provinces and examined for their possible infection by hop stunt viroid (hsvd). the experimental host range of the viroid was determined by injection of infected sap into greenhouse grown seedlings and subsequent assay of inoculated plants by rt-pcr. fig, mulberry and citrus isolates of the viroid were compared with respect to hos...

Abstract Background and Aims: Hop stunt viroid (HSVd, genus Hostuviroid, family Pospiviroidae) has a wide host range among trees and herbaceous plants. The objectives of this study were to compare biological and physical characteristics of three isolates of HSVd from mulberry, fig and citrus and search for other hosts of this viroid in Iran. Materials and Methods: Plant samples were col...

Citrus species grown in Japan are affected by several viruses and viroids, including satsuma dwarf virus, citrus vein enation exocortis viroid, other viroids. Recently, a comprehensive review was made on the occurrence, history, research of following three major Japan: tatter leaf virus (apple stem grooving virus), tristeza (Iwanami 2022). As sequel, this study reviews development viroids as we...

Species of bent-toed gecko (Cyrtodactylus) in Vietnam have been described at a rate of nearly four species per year since 2007 mostly based on morphological data. A tool that guides species delimitation will accelerate the rate of documentation, and at a time when the recognition of species greatly benefits conservation. We use DNA barcoding using COI (550 bp) to re-examine the levels of geneti...

background and aims: citrus exocortis disease is naturally limited to citrus plants and causes a serious disease on sensitive rootstocks such as trifoliate orange. the aim of this study was to sample new variants of citrus exocortis viroid from fars trees with yellowing and suberization symptoms and comparison with other isolates of this viroid. materials and methods: a number of symptomatic or...

Viroids are small (246-401 nucleotides), single-stranded, circular RNA molecules that infect several crop plants and can cause diseases of economic importance. Citrus are the hosts in which the largest number of viroids have been identified. Citrus exocortis viroid (CEVd), the causal agent of citrus exocortis disease, induces considerable losses in citrus crops. Changes in the gene expression p...

Figure 1 Figure 2 Citrus fruits are traditional agricultural products in Egypt. Citrus volkameriana is the best rootstock for growing citrus in Egypt’s desert areas (newly reclaimed land). It is far superior to other rootstocks used in Egypt in its resistance to Citrus tristeza virus as well as its tolerance to drought. Constraints to fruit production are mainly related to tree decline caused b...

Prolonged infection of tomato hybrid (Lycopersicon esculentum x Lycopersicon peruvianum) by Citrus exocortis viroid (CEVd) resulted in viroid-like enlarged structures, detected by gel electrophoresis. This population included two new enlarged variants or D-variants, D-87 and D-76, and three transient species or D-forms, D-38, D-40 and D-43. Sequence analyses exposed a locus near the terminal re...

Abstract Citrus exocortis viroid (CEVd), the causal agent of exocortis, is a pathogen that thought to infect all citrus varieties, although it asymptomatic in most. Symptoms develop on susceptible rootstocks, resulting stunting and yield reduction. To aid detection management CEVd, rapid near-field assay was developed using reverse transcription loop-mediated isothermal amplification (RT-LAMP) ...