The feasibility of isolating and manipulating mesenchymal stem cells (MSCs) from human patients provides hope for curing numerous disease and disorders. Recent phenotypic analysis showed heterogeneity of MSCs. A nestin progenitor cell is a subpopulation within MSCs which plays a role in pancreas regeneration during embryogenesis. This study aimed to separate nestin (+) cells from human bone mar...

background: laron syndrome, is a disease that treated by igf-1. this protein is a single chain and has three disulfide bonds. people with laron syndrome have low rates of cancer and diabetes, although they appear to be at increased risk of casual death due to their stature. igf-1 is synthesized by many tissues and is secreted from liver as an endocrine hormone which is transmitted to other tiss...

background : type 1 diabetes mellitus (t1dm) is an autoimmune disease characterized by selective destruction of pancreatic beta cells.   methods: the study included 80 children, 20 of them have t1dm, 40 children were selected from first degree relatives to the same child and 20 healthy children serve as control. body mass index (bmi) was calculated, random blood glucose and glycosylated hemoglo...

Transferring foreign genes into mushroom mediated by Agrobacterium tumefaciens is a standard technique in genetic engineering. Recombinant human insulin has been greatly used in the treatment of type I diabetes. The production of edible mushroom derived insulin should facilitate oral delivery. In this study we used the Agrobacterium tumefaciens mediated transformation method for the transfer an...

glucose and insulin are two important factors in regulation of food intake. also, existence of central glucoreceptors have been determined in human and many of  animal species, in this study the existence of central glucoreceptors and regulative role of glucose in ingestive behavior of broiler cockerels were studied. in this study used from 128 broiler cockerels. at the first, guide cannula imp...

The peptide hormone insulin produced by pancreatic β-cells undergoes post-transcriptional processing before secretion. In particular, C-peptide is cleaved from pro-insulin to generate mature insulin. Here, we introduce a C-peptide-mCherry human iPSC line (HMGUi001-A-8). was generated CRISPR/Cas9 mediated heterozygous insertion of the mCherry sequence into exon 3 locus. We demonstrate that pluri...

Multilamellar vesicles (noisome) of polyoxyethylene alkyl ether surfactants (Brij 52, 72, 76 and 92) were prepared using classic film hydration method. Vesicle formation ability of the surfactants was assessed in presence or absence of cholesterol. All used surfactants formed vesicles in the absence of cholesterol. Recombinant human insulin was used as a model protein drug to investigate encaps...