in this study a nested-pcr assay was optimized for detection of two bvdv biotype of nadl strain. a part of 5' non-coding region of virus, 249 bp in size, was amplified in rt-pcr. pcr product was cloned in a ptz57r/t vector and sequencing results confirmed the specificity of the test. internal primers were designed and a 155 bp dna fragment was amplified in nested-pcr. the sensitivity of rt...

Newcastle disease is one of the main concerns of poultry farmers. Detection of virulent strains of Newcastle disease virus (NDV) has a great impact on control measures against the disease. In this study RT-PCR was optimized in high sensitivity in order to differentiate the virulent from non-virulent NDV isolates directly in tissue homogenates. The vaccinal NDV strain and known field isolates we...

Background and purpose: Proper diagnosis of patients with COVID-19 is one of the challenges in medical centers. RT-PCR is the standard and reference test in diagnosis of patients with COVID-19. This study aimed at accelerating the correct diagnosis of COVID-19 and the diagnostic power of chest CT scan and RT-PCR. Materials and methods: This study was performed in 569 patients with COVID-19 adm...

Backgrounds and Aims: Coxsakievirus B3 (CVB3), one of the six Coxsakievirus B serotypes, is a member of the Enterovirus genus within the Picornaviridae family. CVB3 is an important pathogen of viral myocarditis, which accounts for more than 50% of viral myocarditis cases. The genome of CVB3, like that of other Entroviruses, is a single-stranded, sense, polyadenylated RNA molecule with 7400 nucl...

During 2009-10, real time RT-PCR and conventional RT-PCR techniques Used for detecting BTVs RNA in 310 blood samples. For real time and gel based RT-PCR segment-1 and segment-10 selected as conserve genes to search any BTV strains. Using these methods, 58 (%18.7) and 14 (%4.5) positive samples were detected among the clinically suspected sheep. Sensitivity of both molecular techniques evalua...

during 2004-2006, a total number of 22 tissue samples obtained from poultry flocks suspected to respiratory diseases submitted to avian virology laboratory of faculty of veterinary medicine, were prepared for avian influenza virus (aiv) isolation in embryonated chicken eggs, according to the standard method. rt-pcr was established on tissue samples using specific primers for h9 gene. amplified ...

Background and Aims: Infectious bronchitis virus (IBV) causes an acute, highly contagious respiratory and kidney disease of chickens which results in significant economic losses in commercial broilers, layers and breeders. Rapid identification of IBV serotypes involved in respiratory complex is a problem in the differential diagnosis. Materials and Methods: In this study, a single and mul...

during 2009-10, real time rt-pcr and conventional rt-pcr techniques used for detecting btvs rna in 310 blood samples. for real time and gel based rt-pcr segment-1 and segment-10 selected as conserve genes to search any btv strains. using these methods, 58 (%18.7) and 14 (%4.5) positive samples were detected among the clinically suspected sheep. sensitivity of both molecular techniques evalua...

backgrounds and aims: coxsakievirus b3 (cvb3), one of the six coxsakievirus b serotypes, is a member of the enterovirus genus within the picornaviridae family. cvb3 is an important pathogen of viral myocarditis, which accounts for more than 50% of viral myocarditis cases. the genome of cvb3, like that of other entroviruses, is a single-stranded, sense, polyadenylated rna molecule with 7400 nucl...