نتایج جستجو برای: polymerize chain reaction

تعداد نتایج: 628214  

پایان نامه :وزارت علوم، تحقیقات و فناوری - دانشگاه کردستان - دانشکده کشاورزی 1392

بیماری¬های ویروسی ناشی از نپوویروس¬ها از مخرب¬ترین بیماری¬های ویروسی مو در سراسر جهان به حساب می¬آیند که با نماتدهای خانواده longidoridae منتقل می¬شوند. اغلب این ویروس¬ها دارای میزبان¬های طبیعی متعددی در بین گیاهان زینتی و غیر زینتی، درختان میوه و علف¬های هرز می¬باشند. پیکره اعضای این جنس دو بخشی، جورقطر و به قطر حدود 30 نانومتر می¬باشد. غلظت این ویروس¬ها در گیاه و به ویژه تاک پایین است و در نت...

Journal: :jundishapur journal of microbiology 0
abdolhassan kazemi tropical and infectious disease research center, tabriz university of medical sciences, tabriz, ir iran mehraban falahati department of medical parasitology and mycology, tehran university of medical sciences, tehran, ir iran; corresponding author: mehraban falahati, parasitology and mycology department, school of medicine, tehran university of medical sciences, tehran, ir iran . tel.: +98-2188058653, fax: +98-2188058653, e-mail: ashraf hajipoor tropical and infectious disease research center, tabriz university of medical sciences, tabriz, ir iran abbasali jafari department of medical mycology, school of medicine, yazd university of medical sciences, yazd, ir iran mohammad asghar zadeh tropical and infectious disease research center, tabriz university of medical sciences, tabriz, ir iran

results 162 yeast species from 250 specimens were isolated in sabroud dextrose agar (sda) medium. 106 (65.4%) of them were germ tube formation positive, 86 (53%) chlamydoconidium formation positive and 101(62.4%) were pcr positive. yeast cells and mycelia were detected in the isolates on direct microscopic examination. c. albicans accounted for 66.04% of cases and 34% were non-c. albicans speci...

Journal: :journal of comprehensive pediatrics 0
mohammad pedram thalassemia and hemoglobinopathy, research center, ahvaz jundishapur university of medical sciences, ir iran +98-6113743285, [email protected]; thalassemia and hemoglobinopathy, research center, ahvaz jundishapur university of medical sciences, ir iran +98-6113743285, [email protected]سازمان های دیگر: thalassemia and hemoglobinopathy, research center morteza heidari department of pediatrics, golestan hospital, ahvaz jundishapur university of medical sciences, ir iranسازمان اصلی تایید شده: دانشگاه علوم پزشکی جندی شاپور اهواز (ahvaz jundishapur university of medical sciences) bijan keikhaei thalassemia and hemoglobinopathy, research center, ahvaz jundishapur university of medical sciences, ir iran +98-6113743285, [email protected]سازمان اصلی تایید شده: دانشگاه علوم پزشکی جندی شاپور اهواز (ahvaz jundishapur university of medical sciences)سازمان های دیگر: thalassemia and hemoglobinopathy, research center reza azizi malamiri department of pediatrics, golestan hospital, ahvaz jundishapur university of medical sciences, ir iranسازمان اصلی تایید شده: دانشگاه علوم پزشکی جندی شاپور اهواز (ahvaz jundishapur university of medical sciences) behzad poopak department of medical sciences, tehran islamic azad university of medical sciences, ir iranسازمان اصلی تایید شده: دانشگاه علوم پزشکی جندی شاپور اهواز (ahvaz jundishapur university of medical sciences) kiavash fekri department of pediatric, shafa hospital, ahvaz jundishapur university of medical sciences, ir iranسازمان اصلی تایید شده: دانشگاه آزاد اسلامی علوم و تحقیقات (islamic azad university science and research branch)

background neuroblastoma is the most common extracranial malignant solid tumor in children under 5 years, and it is characterized by wide clinical and biological heterogeneity. n-myc oncogene amplification is considered to be one of the most important prognostic factors used to evaluate survival in these patients. objectives the aim of our study was to determine amplification of the n-myc oncog...

پایان نامه :وزارت علوم، تحقیقات و فناوری - دانشگاه تربیت مدرس - دانشکده علوم زیستی 1393

آنزیم های dna ligase پایدار به حرارت به علت قابلیت های خود از اهمیت بالایی در زیست شناسی مولکولی برخوردارند. در این بین، آنزیم dna ligase از باکتری thermus thermophilus (tth dna ligase) با قابلیت های ویژه ی خود من جمله پایداری بالای حرارتی و دقت بالای عملکرد، می تواند کاربردهای وسیعی را در تکنیک های تشخیصی بیماری های ژنتیکی مانند تکنیک lcr (ligation chain reaction) و mlpa (multiple ligation-dep...

Journal: :Journal of the American Chemical Society 2017
Emre H Discekici Athina Anastasaki Revital Kaminker Johannes Willenbacher Nghia P Truong Carolin Fleischmann Bernd Oschmann David J Lunn Javier Read de Alaniz Thomas P Davis Christopher M Bates Craig J Hawker

A highly efficient photomediated atom transfer radical polymerization protocol is reported for semi-fluorinated acrylates and methacrylates. Use of the commercially available solvent, 2-trifluoromethyl-2-propanol, optimally balances monomer, polymer, and catalyst solubility while eliminating transesterification as a detrimental side reaction. In the presence of UV irradiation and ppm concentrat...

Journal: :jundishapur journal of microbiology 0
than leslie thian lung department of medical microbiology and parasitology, faculty of medicine and health sciences, university putra malaysia, selangor, malaysia chong pei pei department of biomedical sciences, faculty of medicine and health sciences, university putra malaysia, selangor, malaysia ng kee peng department of medical microbiology, faculty of medicine, university of malaya, lumpur, malaysia. seow heng fong department of pathology, faculty of medicine and health sciences, university putra malaysia, selangor, malaysia; department of pathology, faculty of medicine and health sciences, university putra malaysia, seri kembangan, malaysia, malaysia. tel: +60-389464203, fax: +60-389464232

results all eight candida species were successfully detected, identified and quantitated based on the icl gene. a seven-log range of the gene copy number and a minimum detection limit of 103 copies were achieved. conclusions a one-tube absolute quantification real-time pcr that differentiates medically important candida species via individual unique melting temperature was achieved. analytical ...

Journal: :jundishapur journal of microbiology 0
parisa mohammadi department of microbiology, faculty of biological sciences, alzahra university, tehran, ir iran; department of microbiology, faculty of biological sciences, alzahra university, tehran, ir iran. tel: +98-2185692714, fax: +98-2188058912 aida hamidkhani department of biotechnology, faculty of biological sciences, alzahra university, tehran, ir iran ezat asgarani department of biotechnology, faculty of biological sciences, alzahra university, tehran, ir iran

results the results showed that nl1-gc/ls2 primer set could yield species-specific amplicons, which were well distinguished and allowed better species discrimination than that generated by the its3-gc/its4 primer set, in both dgge and ttge profiles. all five candida species were discriminated by dgge and ttge using the nl1-gc/ls2 primer set. conclusions comparison of dgge and ttge profiles obta...

Journal: :jundishapur journal of microbiology 0
alisha akya nosocomial infection research centre, school of medicine, kermanshah university of medical sciences, kermanshah, ir iran; nosocomial infection research centre, school of medicine, kermanshah university of medical sciences, kermanshah, ir iran. tel: +98-8334274618, fax: +98-8334276477 kamal ahmadi msc student of medical microbiology, school of medicine, kermanshah university of medical sciences, kermanshah, ir iran shahram zehtabian school of medicine, kermanshah university of medical sciences, kermanshah, ir iran afsaneh salimi msc student of medical microbiology, school of medicine, kermanshah university of medical sciences, kermanshah, ir iran azam elahi msc student of medical microbiology, school of medicine, kermanshah university of medical sciences, kermanshah, ir iran sayed hamid madani school of medicine, kermanshah university of medical sciences, kermanshah, ir iran

background aseptic meningitis is the most common type of meningitis and is characterized by meningeal inflammation that is not linked to identifiable bacterial pathogens in cerebrospinal fluid (csf). objectives this study aimed to evaluate the frequency of aseptic meningitis caused by herpesviruses, namely herpes simplex types i and ii (hsv-1, hsv-2), epstein-barr virus (ebv), cytomegalovirus (...

Journal: :jundishapur journal of microbiology 0
ebrahim badparva department of parasitology and mycology, razi herbal medicines research center, lorestan university of medical sciences, khorramabad, ir iran javid sadraee department of parasitology, tarbiat modares university, tehran, ir iran farnaz kheirandish department of parasitology and mycology, razi herbal medicines research center, lorestan university of medical sciences, khorramabad, ir iran; department of parasitology and mycology, razi herbal medicines research center, lorestan university of medical sciences, p. o. box: 381351698, khorramabad, ir iran. tel/fax: +98-6616200133

conclusions in the present study, the highest prevalence was for st5, which is so important for epidemiology and risk of human infection. the report related to st3 in cattle as a subtype of human showed mutual infection between human and cattle. another important point in this study was the st6 report. finally, it seems that gathering epidemiological data is needed for a better understanding of...

Journal: :jundishapur journal of microbiology 0
mahmoud rahdar department of parasitology, faculty of medicine, ahvaz jundishapur university of medical sciences, ir iran ali reza samarbaf-zadeh department of virology, faculty of medicine, ahvaz jundishapur university of medical sciences, ir iran leila arab mycoparasitology department of medical faculty, ahvaz jundishapur university of medical sciences, ir iran +98-9166524492, [email protected]; mycoparasitology department of medical faculty, ahvaz jundishapur university of medical sciences, ir iran +98-9166524492, [email protected]

background toxoplasma gondii is an obligate, intracellular parasite, which is widely spread in the world. the parasite is able to infect all warm-blooded hosts including human.the infection occures via consumption of food or water containing oocytes, eatingundercooked meats containing tissue cysts, and placenta. undercooked meat consumption is one of the most important ways of toxoplasma transm...

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