morphological and electrophoretic analysis of the crystalline inclusions (parasporal crystals) of sporulated cultures of b. thuringiensis ssp. kumamotoensis dsm 6070 (bt 6070) was conducted via phase contrast and scanning electron microscopy and sodium dodecyl sulphate-polyacrylamide gel electrophoresis. the activity of the spore-crystal suspensions against house fly (musca domestica) larvae wa...

We report the draft genome sequence of Bacillus thuringiensis strain LM1212, which differentiates into crystal producers or spore formers during the stationary phase. Availability of this genome sequence will facilitate the study of spore formation, crystal formation, cell differentiation, and evolution of B. thuringiensis.

We developed a process of producing a dead-spore Bacillus thuringiensis Berliner var. kurstaki HD-1 formulation, Toarow CT, and studied several aspects t o improve the productivity of crystal toxin and the insecticidal potency after sterilization to kill spores. The production of cell mass or crystal toxin was highly influenced by aeration and agitation of the culture. The culture broth, within...

objective(s): the aim of the present study is to determine the effect of chloramphenicol (cap) on rat liver and small intestine. effect of phenobarbital (pb) on cap toxicity was also investigated.   materials and methods:   rats were received cap at doses of 0, 200, 400 and 600 mg/kg. another group was pretreated with 80 mg/kg pb 30 min prior to administration of various doses of cap. the exper...

The crystal structure of cyclic AMP-catabolite activator protein (CAP) from Escherichia coli containing cobalt(II) chloride and ammonium sulfate is reported at 1.97 Å resolution. Each of the two CAP subunits in the asymmetric unit binds one cobalt(II) ion, in each case coordinated by N-terminal domain residues His19, His21 and Glu96 plus an additional acidic residue contributed via a crystal co...

Fowler's bacillus is one of several organisms which form a non-viable inclusion or parasporal body during the process of sporulation. This body is globular and may be as large as or larger than the spore. Its position in the cell is not random; the spore is terminal and the body paracentral, lying between the spore and the remaining vegetative cell chromatin bodies. On completion of sporulation...

We studied the effects of combinations of Bacillus thuringiensis spores and toxins on the mortality of diamondback moth (Plutella xylostella) larvae in leaf residue bioassays. Spores of B. thuringiensis subsp. kurstaki increased the toxicity of crystals of B. thuringiensis subsp. kurstaki to both resistant and susceptible larvae. For B. thuringiensis subsp. kurstaki, resistance ratios were 1,20...

  Objective(s): The aim of the present study is to determine the effect of Chloramphenicol (CAP) on rat liver and small intestine. Effect of phenobarbital (PB) on CAP toxicity was also investigated.   Materials and Methods:   Rats were received CAP at doses of 0, 200, 400 and 600 mg/kg. Another group was pretreated with 80 mg/kg PB 30 min prior to administration of various doses...

Background and purpose: Cancer is one of the major health challenges in the world. The efficacy of current treatments is low but their side effects are high. Cold atmospheric plasma (CAP) is a new modality for cancer treatment. This study aimed to compare the cytotoxicity effect of CAP on the cell line models of common cancers and normal cells. Materials and methods: In this experimental study...