The aim of this study was to evaluate the effects of progesterone and ovarian stimulation on the development and implantation rate of mouse embryos. Two-cell embryos were collected from superovulated mice and cultured in the presence of different concentrations of progesterone (0, 5, 10 and 20 ng/ml). Also other mice were rendered pregnant in unstimulated, unstimulated progesterone-injected, su...

OBJECTIVE The objective of this study was to investigate the effects of seasonal changes on the superovulation in Black Suffolk ewes, particularly the ovulation rate and embryo quality. DESIGN Black Suffolk ewes were superovulated either in May (n=22) or in September (n=21), 2013. After estrus synchronization with CIDR, the donor ewes were superovulated with PMSG and seven decreasing doses of...

The intensity and duration of sexual behavior in Bos indicus was assessed through the continuous observation of sexual receptivity. Two groups of cows were formed: only synchronized (n = 50) and other group further superovulated (n = 20). An intravaginal implant that released progesterone over 9 d was used. After removing the implant, 25 mg of PGF2α was administered. In the superovulated group,...

The objective of this retrospective study was to evaluate the factors influencing developmental stage of bovine embryos recovered from superovulated dairy cattle 7 d after estrus. From 217 superovulated dairy cows and heifers, 2,211 eggs were recovered, of which 1,495 were classified as transferable embryos based on morphological evaluation of developmental stage and quality. From the evaluated...

The influence of copper upon some physiological parameters and reproduction in ewes was studied. Four groups of animals were investigated: 1/ control ewes (untreated); 2) ewes receiving copper as a supplement over the recommended amount of copper in food (10, 25 or 50 mg Cu/ewe/day); 3/ control, superovulated ewes; and 4) ewes treated with 50 mg copper during one month and then superovulated. A...

During spring, summer and winter seasons, Sistani donor cows, with normal reproductive status, were superovulated and embryos were recovered non-surgically on day 7. Grade A blastocyst embryos were either transferred fresh (spring) or frozen (summer and winter). Recovered embryos during summer and winter were exposed to glycerol and frozen using conventional method. During spring season, recipi...

Hamster spermatozoa, collected from the caput and the cauda of the epididymis, are known to differ in their motility. They do not normally acquire fertilizing ability until they reach the proximal portion of the cauda epididymidis. The aim of the present investigation was to test the fertilizing capacity of spermatozoa from the caput epididymis after initiation. Sperm cells were incubated by ad...

during spring, summer and winter seasons, sistani donor cows, with normal reproductive status, were superovulated and embryos were recovered non-surgically on day 7. grade a blastocyst embryos were either transferred fresh (spring) or frozen (summer and winter). recovered embryos during summer and winter were exposed to glycerol and frozen using conventional method. during spring season, recipi...

Bovine Embryo transfer has been used widely to reproduce the most valuable females in the herd, with about 750,000 embryos produced annually from superovulated donors and more than 450,000 embryos produced using in vitro techniques. Furthermore, embryos are the safest and most cost effective alternatives to move genetics internationally because of their low risk of transmitting diseases. One of...

Background: Currently, ARTs are of indispensable importance in treatment of human infertility. While ARTs are considered to be safe, some studies suggest that ARTs manipulations may perturb the epigenetic information and subsequently the health of the offspring. To examine these events further, this study was conducted to assess the effect of superovulation, in vitro culture and vitrification o...