The objective of this study was to evaluate, using an embryo donation model, whether impaired oocyte/embryo developmental competence and/or changes in uterine milieu are responsible for the previously observed adverse effects of superovulation with gonadotrophins on implantation and fetal development in mice. Embryos from superovulated and non-stimulated females were transferred to separate ute...

To determine similarities and differences between nonsuperovulated and superovulated ewe models, data collected from several experiments (1989 through 2005) were analyzed. Mature non-pregnant non-superovulated (n = 91) or superovulated (n = 299) Western range-type ewes were used for evaluation of luteal function. To induce superovulation, ewes were injected twice daily with FSH on days 13 to 15...

BACKGROUND The prairie vole (Microtus ochrogaster) is an emerging animal model for biomedical research because of its rich sociobehavioral repertoire. Recently, lentiviral transgenic technology has been used to introduce the gene encoding the green fluorescent protein (GFP) into the prairie vole germline. However, the efficiency of transgenesis in this species is limited by the inability to rel...

Although studies have demonstrated reasonable pregnancy rates in single-ovulating heifers inseminated with sexed sperm, it is not yet clear if sexed sperm can be successfully used in superovulated females. Therefore, the objective of this trial was to determine fertilization rate and embryo quality in superovulated heifers inseminated with frozen-thawed Xchromosome bearing sperm at 2 different ...

MANALU, W. 1999. The effect of superovulation prior to mating on fetal growth in Iambs from Javanese thin-tail ewes. Jurnal Ilmu Ternak dan Veteriner 4 (4): 243-250. Twenty-nine fetuses (11 fetuses from 9 non-superovulated ewes and 18 fetuses from 8 superovulated ewes) were used to study the effect of superovulation of ewes prior to mating on fetal weight, fetal length, the length of the body a...

Mammalian eggs are surrounded by a thick extracellular coat, the zona pellucida, that plays important roles during early development. The mouse egg zona pellucida is constructed of three glycoproteins, called mZP1, mZP2, and mZP3. The gene encoding mZP3 is expressed only by growing oocytes during a 2- to 3-week period of oogenesis. Here, the mZP3 gene was disrupted by targeted mutagenesis using...

Embryo transfer is a multi-step process that involves the production and collection of preimplantation embryos from genetically superior females (called donors) and the subsequent transfer of the harvested embryos into reproductively healthy females (called recipients) for the purpose of establishing pregnancies and producing live offspring. The first successful embryo transfer in mammals was p...

Alternative methods to the utilization of laboratory animal blood and its by-products are particularly attractive, especially regarding hamsters due to their small size and difficulties in obtaining serial blood samples. Steroid hormone metabolite quantification in feces, widely used in studies of free-ranging or intractable animals, is a non-invasive, non-stressor, economical, and animal savin...