Abstract Virus-induced gene silencing (VIGS) is a useful tool to investigate the role of genes particularly in hard-to-transform plant species via Agrobacterium -mediated genetic transformation process. Soybean one most important crops for food and protein source, but its low efficiency makes it hard identify functions interest. Here, we adopted tobacco ringspot virus (TRSV)-based VIGS systems ...

Background: The potential of plants, as a safe and eukaryotic system, is considered in the production of recombinant therapeutic human protein today; but the expression level of heterologous proteins is limited by the post-transcriptional gene silencing (PTGS) response in this new technology. The use of viral suppressors of gene silencing can prevent PTGS and improve transient expression level ...

Virus-induced gene silencing (VIGS) is one of the reverse genetics tools for analysis of gene function that uses viral vectors carrying a target gene fragment to produce dsRNA which trigger RNA-mediated gene silencing. There are a number of viruses which have been modified to silence the gene of interest effectively with a sequence-specific manner. Therefore, different types of methodologies ha...

Short-interfering RNAs (siRNAs), the molecular markers of posttranscriptional gene silencing (PTGS), are powerful tools that interfere with gene expression and counter virus infection both in plants and animals. Here, we report the effect of temperature on geminivirus-induced gene silencing by quantifying virus-derived siRNAs and by evaluating their distribution along the virus genome for isola...

As an allohexaploid plant, common wheat has a complex gene structure, making it difficult to study its function. Virus-induced silencing (VIGS) is important tool for the rapid analysis of plant In this study, system, namely, barley stripe mosaic virus (BSMV)-VIGS induced by BSMV was used silence phytoene desaturase (TaPDS) and actin depolymerization factor (TaADF7) genes determine effect on whe...

background: the potential of plants as a safe and eukaryotic systems, are considered for producing recombinant therapeutic human protein today; but the expression level of heterologous proteins is limited by the post-transcriptional gene silencing (ptgs) response in this new technology. the use of viral suppressors of gene silencing can prevent ptgs and improve transient expression level of for...