A panel of microsatellites mapped to the Leishmania genome might make it possible to find associations between specific loci and phenotypic traits. To identify such loci, a Perl programme was written that scans the sequence of a genome and writes all loci containing microsatellites to a MySQL database. The programme was applied to the sequences of the L. braziliensis, L. infantum and L. major g...

  Abstract   Background: The polymorphic variants at codon 72 of the p53 gene, encoding either   proline or arginine at residue 72, produce marked change in the structure of p53.   From the evidence that the DNAmismatch repair system and p53 interact to maintain   genomic integrity, we hypothesized that the codon 72 variation may influence the   prevalence of microsatellite instability a featur...

Microsatellite markers containing simple sequence repeats (SSRs) are a valuable tool for genetic analysis. Our objective was to identify microsatellite markers that could be used to differentiate between male and female date palm (Phoenix dactylifera). The date palm is a dioecious plant whose sex cannot be determined until it reaches a reproductive age between 5 and 10 years. An early selection...

A series of techniques are presented to construct genomic DNA libraries highly enriched for microsatellite DNA loci. The individual techniques used here derive from several published protocols but have been optimized and tested in our research laboratories as well as in classroom settings at the University of South Carolina and University of Georgia, with students achieving nearly 100% success....

A series of techniques are presented to construct genomic DNA libraries highly enriched for microsatellite DNA loci. The individual techniques used here derive from several published protocols but have been optimized and tested in our research laboratories as well as in classroom settings at the University of South Carolina and University of Georgia, with students achieving nearly 100% success....

Sequencing. Genomic DNA was extracted from peripheral blood using standard procedures and its quality was monitored using a Nanodrop spectrophotometer. DNA was amplified for CDKN2A, CDK4, MC1R, TP53 and MITF as described previously [13, S1]. PCR products were subjected to automatic sequencing by ABI PRISM 310 genetic analyzer (Applied Biosystems). Microsatellite analysis. The primers used for m...

Microsatellites or simple sequence repeats provide an attractive source of genetic polymorphisms for both mammals and plants (1). Microsatellites can be detected by PCR using minute amounts of starting material and they provide codominant markers with a high degree of allelic polymorphism. However, these markers are obtained through a difficult and labor-intensive procedure. In contrast, RAPD m...