abstract background: improvements in culture media formulations have led to an increase in the ability of sheep embryo in culture throughout the preimplantation period. objective: this study was carried out to evaluate the effects of various concentrations of mem vitamins during in vitro maturation of sheep oocytes and subsequent embryo development. materials and methods: sheep ovaries were col...

Diverse groups of chemicals in culture media are needed for successful bovine oocyte maturation and embryo development during which dramatic cytoplasmic and nuclear reprogramming events take place. In vitro embryo production (IVP) procedures frequently include supplements such as serum and/or co-culture with various types of somatic cells. However, the presence of undefined serum in culture med...

Within the embryology laboratory one of the central components is embryo culture media. The fundamental goal/role of the laboratory and therefore the culture media has been to maintain the inherent viability of the gametes/embryos before replacement to the mother. Over the last 10-15 years there have been major advancements in this area, with culture media developing from simple salt solutions ...

The main factors affecting pregnancy and implantation rates are uterine receptivity, embryo quality, and transfer efficiency. Embryo transfer (ET) is the last step of critically important procedure of in vitro fertilization (IVF) and probably the least successful step in Assisted Reproductive Technology (ART) treatment cascade; though simple in most of the cases, it may pose to be the most diff...

This study assessed the feasibility of using LeY glycan secretion level in human embryos as a method of judging embryo quality. Embryo culture media from patients receiving in vitro fertilization-embryo transfer was collected, and quality scores of embryos were recorded. Secretions of LeY in the culture media in different development stages (from 4-cell to 10-cell), embryos in the same developm...

oocyte maturation and embryo development are controlled by intra-ovarian factors such as steroid hormones. progesterone (p4) exists in the follicular fluid that contributes to normal mammalian ovarian function and has several critical functions during embryo development and implantation, including endometrial receptivity, embryonic survival during gestation and transformation of the endometrial...

introduction: determination of best culture medium for fertilization and development of nmri mice. material and methods: development of fertilized mature oocytes of nmri mice was studied for five days in several culture media (ksom, t6, m16, czb, g1tm ver3 and g2tm ver3) following hormone therapy with pmsg and hcg. results: the rate of fertilization after 24 hours was 97.4% in ksom, 83.5% in t6...

Abstract In bovine females, the production of embryos derived from oocytes obtained by ovum pick-up (OPU) is becoming a frequent procedure. Collection pre-pubertal animals enables shortening genetic distance, especially in high-value animals. Nevertheless, oocyte and later embryo developmental potential differ between prepubertal adult heifers. The aim this study was to determine possible under...