background and objective: formalin-fixed paraffin-embedded tissues are a valuable source of dna for molecular studies. we designed and optimized an efficient procedure for dna extraction from formalin-fixed paraffin embedded tissues. materials and methods: seventy three blocks of cervical paraffin-embedded tissues were investigated. dna was extracted using 45 minutes boiling in alkaline solutio...

Recently, we demonstrated chromosome instability (CIN) in human cleavage stage embryogenesis following in vitro fertilization (IVF). CIN not necessarily undermines normal human development (i.e. when remaining normal diploid blastomeres develop the embryo proper), however it can spark a spectrum of conditions, including loss of conception, genetic disease and genetic variation development. To s...

Abstract In total 58 Xanthomona s strains isolated from Araceae worldwide, together with 13 other phylogenetically-related Xanthomonas strains, were characterized using multilocus sequence analysis based on concatenated sequences of seven single copy orthologous genes, extracted whole genome sequences. The revealed a monophyletic clade 48 44 Anthurium , identified as X. phaseoli pv. dieffenbach...

Sanana Honey is a local brand, type of honey produced by forest bees which well known in the Sula Islands, North Maluku. The Islands have potential to be developed as producer quality honey. At this time there has been no scientific report regarding types and characteristics bee species from Islands. This study aims identify honey-producing applying LCO gene DNA barcode. methods used used: 1) e...

Methylated DNA immunoprecipitation (MeDIP) is one of the most widely used methods to evaluate DNA methylation on a whole genome scale, and involves the capture of the methylated fraction of the DNA by an antibody specific to methyl-cytosine. MeDIP was initially coupled with microarray hybridization to detect local DNA methylation enrichments along the genome. More recently, MeDIP has been coupl...

Background: Gossypium thurberi is a wild diploid species that has been used to improve cultivated allotetraploid cotton. G. thurberi belongs to D genome, which is an important wild bio-source for the cotton breeding and genetic research. To a certain degree, chloroplast DNA sequence information are a versatile tool for species identification and phylogenetic implications in plants. Different ch...

Background and Objective: Enterococcal infections are considered the most common nosocomial infections. Nowadays, enterococci show high resistance to common antibiotics, especially vancomycin. Vancomycin-resistant Enterococcus faecium is one of the most common nosocomial infections, which is included in the World Health Organization priority pathogens list for research and development of new an...

The identification of DNA methylation patterns is a common procedure in the study of epigenetics, as methylation is known to have significant effects on gene expression, and is involved with normal development as well as disease. Thus, the ability to discriminate between methylated DNA and non-methylated DNA is essential for generating methylation profiles for such studies. Methylated DNA immun...

This paper examines the model of network genomics pioneered in late 1980s and adopted European Commission-led Yeast Genome Sequencing Project (YGSP). It contrasted with burgeoning large-scale center being developed United States to sequence yeast genome, chiefly as a pilot for tackling human genome. We investigate operation connections two models by exploring co-authorship that captures differe...

5-Hydroxymethylcytosine (5hmC) is a modified base present at low levels in various mammalian cells, and it plays essential roles gene expression, DNA demethylation, genomic reprogramming. Herein, we develop label-free template-free chemiluminescent biosensor for sensitive detection of 5hmC DNAs based on 5hmC-specific glucosylation, periodate (IO4+) oxidation, biotinylation, terminal deoxynucleo...