نتایج جستجو برای: induced pluripotent stem cells
تعداد نتایج: 2212149 فیلتر نتایج به سال:
Whether human induced pluripotent stem cells (hiPSCs) are epigenetically identical to human embryonic stem cells (hESCs) has been debated in the stem cell field. In this study, we analyzed DNA methylation patterns in a large number of hiPSCs (n = 114) and hESCs (n = 155), and identified a panel of 82 CpG methylation sites that can distinguish hiPSCs from hESCs with high accuracy. We show that 1...
Introduction: Amniotic fluid contains a mixture of different cell types sloughed from the fetal skin, respiratory, alimentary and urogenital tracts, as well as the amnion membrane. As amniotic fluid develops prior to the process of gastrulation, many cells found in its heterogeneous population do not undergo lineage specialization. Therefore, amniotic fluid-derived mesenchymal stem cells (AF-MS...
Fibroblast growth factors (FGFs) are essential for maintaining self-renewal in human embryonic stem cells and induced pluripotent stem cells. Recombinant basic FGF (bFGF or FGF2) is conventionally used to culture pluripotent stem cells; however, because of the instability of bFGF, repeated addition of fresh bFGF into the culture medium is required in order to maintain its concentration. In this...
Reprogramming is a dynamic process that can result in multiple pluripotent cell types emerging from divergent paths. Cell surface protein expression is a particularly desirable tool to categorize reprogramming and pluripotency as it enables robust quantification and enrichment of live cells. Here we use cell surface proteomics to interrogate mouse cell reprogramming dynamics and discover CD24 a...
There has been a long persisting dilemma about potential ovarian stem cells in adult mammalian ovaries, including human, and now there is steadily increasing experimental evidence on their existence. After some previous indirect evidence about the presence of stem cells in adult mouse ovaries, an important breakthrough was made by Zou and his co-workers who successfully established long-persist...
objective: human basic fibroblast growth factor (bfgf) plays an important role in cellular proliferation, embryonic development, and angiogenesis as well as in several signaling pathways of various cell types. bfgf is an essential growth factor for the maintenance of undifferentiated human embryonic stem cells (hescs) and human induced pluripotent stem cells (hipscs). materials and methods: in ...
A Need for meat is rising day by and the production of poultry, cows, pigs fisheries reduced as there no good commercial production. Wider livestock becoming unavailable on a global scale in requisites welfare animals, ecological sustenance human health conditions. Choices cultured are being explored using innovative techniques like stem cells, Embryonic Stem Cells, Induced pluri potent cells [...
Reprogramming of somatic cells to induced pluripotent stem cells (iPSCs) is typically an inefficient and asynchronous process. A variety of technological efforts have been made to accelerate and/or synchronize this process. To define a unified framework to study and compare the dynamics of reprogramming under different conditions, we developed an in silico analysis platform based on mathematica...
This paper reports a novel cell culture chip, namely “inverting microwell array chip”, for cultivation of human induced pluripotent stem cells with controlled microenvironment. Our “inverting microwell array chip” comprises a lower hydrogel microwell array and an upper polystyrene (PS) culture surface. We demonstrate the formation of uniform human induced pluripotent stem cell aggregates in the...
Reprogramming of somatic cells into induced pluripotent stem cells (iPSCs) is achieved by viral-mediated transduction of defined transcription factors. Generation of iPSCs is of great medical interest as they have the potential to be a source of patient-specific cells. For the eventual goal of clinical application, it is necessary to overcome the limitations of low reprogramming efficiency and ...
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