background: the definitive endoderm (de) differentiation using the undefined media and non-human feeders can cause contaminations in the generated cells for therapeutic applications. therefore, generating safer and more appropriate de cells is needed. this study compared five different methods to establish an appropriate method for inducing an efficient de differentiation from human induced plu...

background: the use of stem cells is considered as an appropriate source in cell therapy and tissue engineering. differentiation of human induced pluripotent stem cells (hipscs) to hepatocyte-like cells (hlcs) on mouse embryonic fibroblasts (mefs) feeders is confronted with several problems that hinder the clinical applications of these differentiated cells for the treatment of liver injuries. ...

stem cell therapy can be useful for spinal cord injury (sci). induced pluripotent stem cells (ipscs) are generated by reprogramming mature, fully differentiated cells into a pluripotent state. the ability to generate pluripotent cells from adult somatic cells without the need for an embryo was a major development in stem cell biology. ipscs were established from mouse fibroblasts and demonstrat...

personalized medicine as a novel field of medicine refers to the prescription of specific therapeutics procedure for an individual. this approach has established based on pharmacogenetic and pharmacogenomic information and data. the terms precision and personalized medicines are sometimes applied interchangeably. however, there has been a shift from “personalized medicine” towards “precision me...

Induced pluripotent stem cells (iPSCs) are reprogrammed from somatic cells through numerous transcription factors. Human induced pluripotent stem cell approaches are developing as a hopeful strategy to improve our knowledge of genetic association studies and the underlying molecular mechanisms.  Rapid progression in stem cell therapy and cell reprogramming provides compelling reasons for its fe...

Regenerative capacity of mammals is limited and can rarely regenerate a specific organ or tissue fully. Due to these limitations, regenerative medicine seeks efficient and safe cell sources for regeneration of damaged tissues and organs or treatment for incurable diseases. Human embryonic stem cells (HESCs) hold two important properties called self renewal and pluripotency. However, the use of ...

Background: We have recently shown that human embryonic (hESCs) and induced pluripotent stem cells (hiPSCs) can differentiate into advanced spermatogenic cells including round spermatids by in vitro culture (Easley et al., Direct differentiation of human pluripotent stem cells into haploid spermatogenic cells. Cell Reports 2, 440-446 2012) and also, in collaboration, that rhesus spermatogonial ...

spinal cord injury (sci) is a destructive event that often lead to permanent neurologic deficit. current clinical treatments are aimed at preventing secondary damage, promoting regeneration, and replacing destroyed spinal cord tissue, although effective treatments for sci remain limited. cell therapies for treating sci are promising therapy for replacing dead cells, neuroprotection and axon reg...