The trabecular meshwork and scleral spur of two young human eyes were studied by light and electron microscopy in Epon-embedded material. Anteroposterior sections showed, in light microscopy, numerous dots stained dark purple with Weigert's elastic stain and greenish with Giemsa in both regions. In tilted frontal sections, similarly stained, long, circularly oriented fibers toere observed, indi...

Immunopathological investigations of the small round cells infiltrating into the tissues of human renal allografts and also those in urinary sediments were performed, using anti-human T-lymphocyte serum (AHTS). AHTS made from ALS is highly specific for T cells, but not for B cells. Tissues obtained by needle biopsy were examined by immunofluorescent staining and conventional histopathological m...

Cytologic diagnosis of mycobacterial infection has conventionally depended on the recognition of granulomatous inflammation with caseous necrosis and the identification of acid-fast bacilli with special stains. Immunocompromised patients however may not mount the expected response. Mycobacteria can be seen as negative images in fine needle aspiration cytologic smears from patients with acquired...

Background Assessment of erythrocytes and leucocytes in thin blood films can be used as an inexpensive diagnostic aid in a series of disease states, e.g. infections, anemia and hematological malignancies. Manual counting of cells is still considered the gold standard for example to establish the level of parasitemia in malaria. However, manual cell counting is time consuming and subject to vari...

Skin lesions were found in two 3–4-year-old Holstein cows in a dairy farm in Shiraz. Lesions were distributed mostly around the neck, forelimbs, hindlimbs, and bilateral of midline portion and varied from nodule-like structure to patch with extensive accumulation of crust. Skin scraping samples were taken from each animal and direct smears were prepared, stained with Giemsa and observed under a...

We have compared results of Plasmodium species identification obtained with conventional on-site microscopy of Giemsa-stained thick smears (GTS) and a semi-nested polymerase chain reaction (PCR) in 96 malaria patients from Rondônia, Western Brazilian Amazon. Mixed-species infections were detected by PCR in 30% patients, but no such case had been found on GTS. Moreover, P. malariae infections we...

Equine piroplasmosis is a tick-borne disease caused by intra-erythrocyte protozoa, Theileria equi and Babesia caballi. The present study aimed to detect piroplasm infection in horses in Kurdestan Province, Iran, through molecular and microscopic approaches.n this study, 186 blood samples were randomly collected from horses of five regions of Kurdestan Province. The Tbs-S/Tbs-A primer set was us...