نتایج جستجو برای: cloning vector
تعداد نتایج: 249528 فیلتر نتایج به سال:
Abstract Background and Objectives In Iran, Khorasan province is an endemic area for HTLV-1 virus. Considering the inability of serological tests to determine HTLV-1 in window period, their failure to confirm the indetermination results of western blot, and given the probability for HTLV-1 transfusion transmission, a SYBR green-based Real Time PCR was set to measure the HTLV-1 proviral load. ...
due to the widespread applications of xylitol dehydrogenase, an enzyme used for the production of xylitol, the present study was designed for the cloning of xylitol dehydrogenase gene from glcunobacter oxydans dsm 2003. after extraction of genomic dna from this bacterium, xylitol dehydrogenase gene was replicated using polymerase chain reaction (pcr). the amplified product was entered into ptz5...
Background and Objective: Breast cancer is the fifth common cause of death in the world and is considered as the leading cause of cancer related death among females. HER2 is a surface antigen which is overexpressed in 30% of breast cancer cases. The aim of this study was cloning of HER2 receptor gene in pCDNA3.1Hygro+ expression vector, expression of this protein in LL2 eukaryotic cell line and...
Toxoplasma gondii contain various immunogenic antigens. The most important Toxoplasma antigens are somatic and excreted/secreted antigens. Rhoptry proteins are known as excreted/secreted antigens. These antigens have been proposed as a vaccine candidate against toxoplasmosis. The main objective of the present work was cloning rhoptry protein1 (ROP1) Gene of Toxoplasma gondii (RH) in a cloning...
Background and purpose: Toxoplasmosis is a common parasitic disease throughout the world and one-third of the population has antibodies to Toxoplasma gondii. This disease causes serious medical problems in fetuses and immunocompromised individuals. As gene encoding protein GRA14 can be considered as a suitable target for DNA vaccine and designing diagnostic kits the aim of this study was to ...
Introduction:Toxoplasma gondii is a ubiquitous obligate intracellular parasite with a relatively broad host range infecting both mammals and birds. Toxoplasma proteins are strong antigens that can begin strong immune reactions, among which Rhoptry protein 1 (ROP1) can be named discharging from rhoptry cell-organ. ROP1 is regarded as a competitor for recombinant vaccines against toxoplasmosis. T...
We have developed an efficient strategy for cloning of PCR products that contain an unknown region flanked by a known sequence. As with ligation-independent cloning, the strategy is based on homology between sequences present in both the vector and the insert. However, in contrast to ligation-independent cloning, the cloning vector has homology with only one of the two primers used for amplific...
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toxoplasma gondii contain various immunogenic antigens. the most important toxoplasma antigens are somatic and excreted/secreted antigens. rhoptry proteins are known as excreted/secreted antigens. these antigens have been proposed as a vaccine candidate against toxoplasmosis. the main objective of the present work was cloning rhoptry protein1 (rop1) gene of toxoplasma gondii (rh) in a cloning v...
Abstract Generation of new DNA constructs is an essential process in modern life science and biotechnology. Modular cloning systems based on Golden Gate cloning, using Type IIS restriction endonucleases, allow assembly complex multipart from reusable basic parts a rapid, reliable automation-friendly way. Many such toolkits are available, with varying degrees compatibility, most which aimed at s...
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