To explore the potential for in vitro rapid regeneration of Sainfoin (Onobrychis sativa), different concentrations of 6Benzylaminopurine (BAP), 1-naphthaleneacetic acid (NAA) and BAP combined with Indole-3-butyric acid (IBA) were evaluated for their effects on the induction of somatic embryos from leaf, stem and root explants. Different explants were cultured on MS medium supplemented with vari...

Buds from the branches cut during dormancy (end of January) were used as the initial explants and set to develop under laboratory conditions at room temperature. Aseptic culture was established on Murashige and Skoog (MS) medium with BA 2.0, IBA 0.5 and GA3 0.1 mg 1-1. MS media with BA and IBA or NAA and GA3 were used for multiplication phase, whereas medium MS with mineral salts reduced to 1/2...

An efficient protocol providing a dual regeneration pathway via direct shoot organogenesis and somatic embryogenesis for an endangered species, Metabriggsia ovalifolia W. T. Wang, was established from leaf explants. When applied at 2.5 μM, the cytokinins 6-benzyladenine (BA) or thidiazuron (TDZ) and the auxins indole-3-butyric acid (IBA), α-naphthaleneacetic acid (NAA) and indole-3-acetic acid ...

The present study was conducted at the Biotechnology Laboratory, Biotechnology Division, Bangladesh Agricultural Research Institute (BARI), Gazipur during the period from September 2004 to June 2005 to investigate the effect of different concentrations of BAP and NAA on virus free plant regeneration, shoot multiplication and different concentrations of IBA and IAA on in vitro root formation of ...

Effects of indole acetic acid (IAA), indole butyric acid (IBA) and naphthalene acetic acid (NAA) were investigated on some physiological parameters in detached leaves of Tropaeolum majus L., during dark-induced senescence. Auxin accelerated the loss of chlorophyll (Chl) and carotenoid content whereas it retarded the loss of protein amount at the end of senescing period in leaves significantly. ...

This study analyzed the potential of producing Melissa officinalis L. using stem cuttings. Four different hormones (IAA, IBA, NAA, and GA3) were applied to the cuttings, with and without buds, in two doses (1000 mg/L and 5000 mg/L), and after 60 days, 10 morphological characteristics of newly generated plants were detected, and a statistical analysis was carried out. The results of the study sh...

BACKGROUND AND AIMS Cymbidium mastersii is an epiphytic orchid distributed mainly in Northeast India. Owing to its high commercial value in the floricultural industry, natural populations are under threat from over-exploitation. Mass propagation provides an alternative means of satisfying the demand. Unfortunately, conventional propagation is slow and difficult, suggesting in vitro methods for ...