in vitro propagation of az x m9 new apple rootstock resulted of breeding program of vegetative apple rootstock in iran was investigated. nodal explants were inoculated in murashige and skoog (ms) medium supplemented with various concentrations of 6-benzylaminon purine (bap) (0.4, 0.8 and 1.2 mg/l) alone or with naphthalene acetic acid (naa) (0.1 mg/l). we examined the effect of different concen...

background and objectives:  haplophyllum canaliculatum isan endemicand endangerediranian plant from rutaceae family. the object of this work was to study the volatile production in established shoot and callus cultures of haplophyllum canaliculatum as well as isolation, identification and sequencing of 18s rrna gene from callus culture. methods: shoot and callus cultures of h. canaliculatum wer...

The aim of the present study was to investigate the effect of follicle stimulating hormone (FSH) on survival, activation and growth of ovine primordial follicles using histological studies. Pieces of ovine ovarian cortex were obtained and cultured for 6 days in Minimum Essential Medium supplemented with or without FSH (50 ng/mL). These fragments were then process to be used for histology compar...

Background Melatonin (N-acetyl-5- methoxytryptamine) is mainly synthesized and secreted in the pineal gland, ovary, testes, bone marrow, retina and lens in mammalian species. It is involved in the detoxification of ROS and protects embryos from oxidative damage. Melatonin acts as a potential free radical scavenger, including peroxyl radical and hydroxyl radical. In addition, it can stimulate th...

a procedure was developed for rapid in vitro germination of linum album seeds by scarification and exposing to gibberellic acid (ga3) and kinetin (kn).  concentrations of podophyllotoxin (ptox) and related aryltetralin lignans α-peltatin, β-peltatin, 5’-demethoxy-6-methoxypodophyllotoxin and 6-methoxypodophyllotoxin (mptox) in l. album fresh plant organs were determined by hplc. a degree of var...

background: linoleic acid (la) is a polyunsaturated fatty acid present in high concentrations in follicular fluid, when added to maturation culture media, it affects oocyte competence.objective: in the present study, we investigated effect of linoleic acid supplementation on in vitro maturation, embryo development and apoptotic related gene expression in ovinematerials and methods: the experime...

Plantlets grown in conventional tissue culture systems usually encounter physiological and anatomical abnormalities including inability to photosynthesize, low chlorophyll content, open stomata, lack of a cuticle layer in the leaf, abnormal xylem parenchyma etc. Photoautotrophic and photomixotrophic systems could diminish these problems. The purpose of this study was to increase the chlorophyll...