Background & Aim: Considering that antioxidants are known as effective free radicals scavenger, it may be able to improve the in vitro oocyte maturation and the fetal quality. This study was designed to determine the effect of Gundelia tournefortii leaves (GTE) hydro-alcoholic extract as a source of antioxidant on in vitro oocyte maturation. Experimental: Germinal vasicle (GV) were...

Background: In vitro culture of ovarian follicles may preserve fertility in women with premature ovarian failure due to cancer .It seems that creation a condition that could maintain cellular communications and supports growth of follicles to produce mature oocytes appear to be essential. Nitric oxide (NO) has been recently shown to act with a dual action in mouse oocyte meiotic maturation depe...

background: oocyte maturation and subsequent in vitro production (ivp) of embryos are affected by diverse groups of chemicals in maturation medium which are needed for successful mammalian oocyte maturation during which the dramatic cytoplasmic and nuclear reprogramming events take place. this study was designed to evaluate the effects of protein source (fetal bovine serum, fbs, and bovine seru...

background: in general, 15% of oocytes collected in art cycles are immature. these oocytes may be cryopreserved further for use in in-vitro maturation (ivm) program. objective: the aim of this study was to determine maturation capacity, morphometric parameters and morphology of human immature oocytes in both fresh ivm (fivm) and vitrified-ivm (vivm) oocytes. materials and methods: 93 women who ...

  Background & Aim: Considering that antioxidants are known as effective free radicals scavenger, it may be able to improve the in vitro oocyte maturation and the fetal quality. This study was designed to determine the effect of Gundelia tournefortii leaves (GTE) hydro-alcoholic extract as a source of antioxidant on in vitro oocyte maturation. Experimental: Germinal vasicle (GV) were...

Objective:In vitro maturation is a highly effective method for achieving mature  oocytes to treat infertility based on in vitro methods (IVF-ICSI). This technique is effective for treating women who suffer from polycystic ovary syndrome (PCO) and cancer. MATERIALS AND METHODS: immature oocytes were obtained from 6-8 weeks old NMRI mice, 48 hours after intraperitoneal injection of 7.5 units of...

objective: currently, there is no agreement on the optimal urinary derived human chorionic gonadotropin (u-hcg) dose requirement for initiating final oocyte maturation prior to oocyte collection in in vitro fertilization (ivf), but doses that range from 2500- 15000 iu have been used. we intended to determine whether low dose u-hcg was effective for oocyte maturation in ivf/intracytoplasmic sper...

Background: Since the freezing method was offered, much effort has been done to improve the freezing conditions in different studies and the impact of different freezing methods and mediums on oocyte, embryo and ovarian tissues has been examined. Based on studies, minerals play an important role in oocyte maturation and subsequent embryo development. Vanadium is one of the minerals, which is ve...

The cryopreservation of immature GV oocytes by vitrification is offering attractive perspectives, since they represent an important pool of germ cells for women undergoing IVM cycles, or at risk of infertility for OHSS or PCOs, particularly those poor responders to gonadotropin stimulation, or in case of cancer. Therefore, it becomes necessary to optimize both cryopreservation technique as well...