The effect of pretreatment of rats with 5-fluorouracil (5-FU) on hepatic drug-metabolizing enzyme activities was examined. Male rats, weighing 165g, were given a single i.p. injection of 5-FU, 120 mg/kg, and 7 days later the drug-metabolizing enzyme activities of a 10,000 x g supernatant fraction from liver homogenates were exam ined. Aniline hydroxylase, ethylmorphine /V-demethylase, and 2-(me...

The effect of pretreatment of rats with 5-fluorouracil (5-FU) on hepatic drug-metabolizing enzyme activities was examined. Male rats, weighing 165g, were given a single i.p. injection of 5-FU, 120 mg/kg, and 7 days later the drug-metabolizing enzyme activities of a 10,000 x g supernatant fraction from liver homogenates were exam ined. Aniline hydroxylase, ethylmorphine /V-demethylase, and 2-(me...

Liv.52 syrup, an Ayurvedic liver-tonic and extract of Solanum nigrum (one of the constituents of Liv.52) were orally administered (0.125 ml/kg b wt./day which is equal to human dose) to weaning rats for 11 weeks. Liv.52 treated rats exhibited better growth during 3rd to 8th week than those administered extract of S. nigrum or controls, after which period the body weight in all the groups gradua...

Animal studies investigating the efficacy of neurotrophic factors as treatments for Parkinson's disease (PD) ideally require partial dopamine (DA) lesion models. The intrastriatal 6-hydroxydopamine (6-OHDA) lesion model may be suitable for this purpose. Although this model has been well characterized in rodents, it has not previously been used in monkeys. The goal of the present study was to ch...

The effect of chronic ethanol consumption on the ability of isolated liver fractions to metabolize the carcinogen N-nitrosopyrrolidine (NPY) was examined. Microsomal fractions of treated animals exhibited increased rates of alpha-hydroxylation of NPY. Similar increases in the specific activities of aniline hydroxylase, reduced nicotinamide adenine dinucleotide phosphate cytochrome c reductase, ...

Hybridomas were prepared from mouse myeloma cells and spleen cells derived from female BALB/c mice that had been immunized with a partially purified ethanol-induced rat liver cytochrome P-450 (P-450et). Monoclonal antibodies (MAbs) produced by the hybridomas were screened for binding to P-450et with a radioimmunoassay. Thirty-one independent hybrid clones produced MAbs that had a high affinity ...

Benzphetamine demethylase and aniline hydroxylase activities were determined with various hemoproteins including indoleamine 2,3-dioxygenase in a cytochrome P-450-like reconstituted system containing NADPH, NADPH-cytochrome P-450 reductase, and O2. The highest specific activities, almost comparable to those of liver microsomal cytochrome P-450, were detected with indoleamine 2,3-dioxygenase fro...

Beagle dogs are commonly used for toxicological and pharmacological studies of drug candidates in the pharmaceutical industry. Recently, we reported a CYP1A2-deficient dog with a nonsense mutation (C1117T). In this study, using CYP1A2-deficient and wild-type dog liver microsomes, substrate specificity of dog CYP1A2 was investigated and compared with human CYP1A2. For this purpose, 11 cytochrome...