AIMS To isolate RNA and DNA simultaneously from formalin fixed paraffin wax embedded tissue to assess the clonality of enteropathy associated T cell lymphomas and to analyse it in detail by a non-radioactive method of T cell receptor complementarity determining region 3 (CDR3) spectratyping. METHODS DNA and RNA were isolated simultaneously from formalin fixed paraffin wax embedded tissue bloc...

BACKGROUND Tissue microarray technology has provided a high throughput means of evaluating potential biomarkers in archival pathological specimens. This study was carried out in order to produce tissue microarray blocks using mechanical pencil tips without high cost. METHOD Conventional mechanical pencil tips (Rotring Tikky II Mechanical Pencil 1.0 mm) were used to cut out 1 mm wax cylinders ...

Abstract Extraction of DNA and RNA from formalin-fixed paraffin-embedded (FFPE) tissue blocks is a critical process in molecular oncology testing. Using FFPE, it possible to choose the portion study, taking into account cell morphology, storage stability conditions at room temperature, make retrospective studies with clinical pathological information. In prostate cancer tissue, contrast macrosc...

Lung carcinomas are characterised by considerable histological variation within the tumour. The possible effects of this morphological heterogeneity on the estimation of tumour ploidy were investigated. Multiple tissue blocks were systematically taken from 20 lung tumours and analysed by flow cytometry. The routine, archival paraffin wax embedded diagnostic blocks from these cases were also ana...

Nowadays, the consumption of meat and meat products has been increased with modern manufacturing techniques. Due to the economic value, the likelihood of using unauthorized tissue is possible in meat products. The aim of this study was to apply morphological methods for detection of unauthorized tissues in meat sausage. In this study, a total number of 20 samples of different types of sausages ...

Plastination is a laboratory process to obtain permanent dry tissue and organ sample. That can be used in the future for investigation educational purposes. This methodology based on dehydration penetration of synthetic substances such as silicon into tissue. In this study, it was aimed deplastinate previously plastinated kidneys order examine them under light microscope. 14 sheep were used, se...