The performance of seed tubers harvested from potato plants grown from sprout cutting, stem cutting and conventional seed tubers against PVY and PLRV were investigated. Sprout cutting, stem cutting and conventional seed tubers of variety Diamant were planted and second and third generation seed tubers were harvested. The least incidence of PVY and PLRV was recorded when seed tubers from sprout ...

Potato leafroll polerovirus (PLRV) genomic RNA acts as a polycistronic mRNA for the production of proteins P0, P1, and P2 translated from the 5'-proximal half of the genome. Within the P1 coding region we identified a 5-kDa replication-associated protein 1 (Rap1) essential for viral multiplication. An internal ribosome entry site (IRES) with unusual structure and location was identified that re...

DNA encoding the coat protein (P3) of a Scottish isolate of potato leafroll virus (PLRV) was inserted into the genome of Autographa californica nucleopolyhedrovirus (AcNPV) such that the coat protein was expressed either in an unmodified form or with the addition of the amino acid sequence MHHHHHHGDDDDKDAMG at the N terminus (P3-6H). Insect cells infected with these recombinant baculoviruses ac...

Eight viruses infect ulluco (Ullucus tuberosus Caldus), an Andean tuber crop. Three of them, potato leafroll virus (PLRV), Andean potato latent virus, and potato virus T, also infect potato (Solanum tuberosum L.). Arracacha virus A and papaya mosaic virus, ulluco isolate (PapMV-U) infect other crops. PLRV, one of the most damaging potato viruses, was detected in healthy potato plants growing in...

The putative RNA-dependent RNA polymerase of potato leafroll luteovirus (PLRV) is expressed by -1 ribosomal frameshifting in the region where the open reading frames (ORF) of proteins 2a and 2b overlap. The signal responsible for efficient frameshift is composed of the slippery site UUUAAAU followed by a sequence that has the potential to adopt two alternative folding patterns, either a structu...

The vast majority of plant viruses are transmitted by insect vectors, with many crucial aspects the transmission process being mediated key protein–protein interactions. Still, very few vector proteins interacting have been identified and functionally characterized. Potato leafroll virus (PLRV) is most efficiently Myzus persicae, green peach aphid, in a circulative, non-propagative manner. Usin...

Potato leafroll virus (PLRV) capsid comprises 180 coat protein (CP) subunits, with some percentage containing a readthrough domain (RTD) extension located on the particle's surface. The RTD N terminus is highly conserved in luteovirids and this study sought to identify biologically active sites within this region of the PLRV RTD. Fourteen three-amino-acid-deletion mutants were generated from a ...

A season-long survey of common weeds was taken near onion and potato fields located within a large vegetable production region in western New York in 2008 and 2009. The objective was to determine the abundance of weed species known as hosts for Iris yellow spot virus (IYSV), a serious pathogen of onion, Potato leafroll virus (PLRV) and Potato virus Y (PVY), which are major pathogens of potato. ...