Proteins not only play an important role in biology, but also have large potential applications in pharmaceuticals and therapeutics, food processing, textiles and leather goods, detergents, and paper manufacturing. With the development of molecular biology technologies, various kinds of proteins can be prepared from upstream processes and from biological raw materials. However, there exist vari...

bacillus thuringiensis (bt) has been extensively used in biopesticidal formulations due to its safe environmental and human health records. the widespread use of bt is often challenged by production as well as formulation costs which are in direct contact with downstream processing, i.e. the separation/purification step. downstream separation/purification efficacy governs the marketability of a...

There are many thousand kinds of proteins with different properties and functions in a cell. To study a protein, one has to obtain the highly purified intact form of the protein. A major protein is not so difficult to be purified while a minor one may need many purification steps and high skills on the techniques. This chapter describes the principles underlying techniques for separation and pu...

background: alkyl hydroperoxide reductase (ahpc) of helicobacter pylori is considered as a diagnostic antigen. there­fore, this antigen can be used to detect h. pylori infection by stool immunoassays such as elisa. the aim of this study was to simplify the ahpc protein purification procedures. methods: for whole cell protein extraction, the bacterial cells were ruptured by octly-β-d glucopyrano...

The rapidly growing field of biotechnology has created a critical need for simple, fast andhigh-throughput processes for the separation and purification of biomolecules from biologicalmatrices. In recent years, several bioseparation techniques have been proposed as advancedalternatives to the classical separation methods. These modern processes emphasize ultrahighselective and sensitive analysi...

With the explosion of proteomics and protein engineering, modern medicine and life science has taken a strong foothold in bench-scale laboratories, both academic and industrial. A vital component of benchscale development of protein-based technologies is purification. Engineered proteins often undergo a stage of the purification process termed ultrafiltration separation. This involves the separ...