background: serological diagnosis of toxoplasma gondii infection using crude antigens may not be more accurate. to increase the diagnostic potency of anti-gens, isolation of their immunogenic fractions could be useful. the current re-search adopted to obtain an affinity isolated fraction from rh strain using cnbr sepharose 4b column coupled with infected mice sera helping in detec-tion of igm a...

In a recent article on the treatment of autoimmune thrombocytopenia (AITP) with anti-D Ig, Bussel et al' make several assertions that we would question. Many preparations of anti-D Ig for intramuscular use contain substantial quantities of polymeric IgG, but this is responsible for most of the inhibitory activity against macrophage Fc receptors and we have shown that the platelet count response...

BACKGROUND Anti-G antibodies are rarely found since anti-D, in combination with anti-C, are difficult to discriminate from anti-G antibodies in routine testing. CASE REPORT A 22-year-old, gravida-3, para-1, woman with blood group A Rh D neg ccddee and known antibody anti-Jk(b), gave birth to her second child. While anti-Jk(b) could not be detected at birth, a new anti-C was found. Antibody sc...

The suppressive effect of IgG on Ab responses to particulate Ags such as erythrocytes is well documented. IgG-mediated suppression is used clinically in rhesus prophylaxis to prevent RhD-negative mothers from becoming immunized against their Rh D-positive fetuses. We have recently shown that IgG anti-SRBC, passively administered together with SRBC, can induce efficient suppression of primary Ab...

Background: Recurrent blood transfusion is a common treatment in patients with thalassemia. The development of antibodies against red blood cell (RBC) antigens complicates RBC cross-matching, enhances the in vivo destruction of transfused cells, accelerates tissue iron overloading, delays the provision of safe transfusion, and reduces health-related quality of life. Materials and Methods: In t...

Clinicians sometimes are confronted with the challenge of transfusing haemorrhaging Rhesus (Rh) D negative patients with Rh D positive blood to save their lives. There are concerns about alloimmunization and future haemolytic disease of the newborn in women of the reproductive age. Another fear is transfusion reaction if they receive another Rh D positive blood in future. We present a 32-year-o...

Rh(D) antigen-containing polypeptide was prepared by immune precipitation of intact cDE/cDE erythrocytes by using a high-titer preparation of polyclonal anti-D. when isolated Rh(D) polypeptide was administered to rabbits, antibody was produced that was unresponsive toward Rh-positive and -negative cells but reacted strongly with the immunogen in enzyme-linked immunosorbent assay-type immunobind...