نتایج جستجو برای: the quantitative real time rt
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Vol. 35, No. 6 (2003) BioTechniques 1141 the ABI PRISM® 7000 and the TaqMan Universal PCR Master Mix protocols (Applied Biosystems). After discovering that the RNA from the thecal cells had vascular endothelial growth factor (VEGF) amplification and that RNA from granulosa cells had no VEGF amplification, the 18S rRNA amplification (TaqMan Pre-Developed Assay Reagents; Applied Biosystems), whic...
SUMMARY Q-Gene is an application for the processing of quantitative real-time RT-PCR data. It offers the user the possibility to freely choose between two principally different procedures to calculate normalized gene expressions as either means of Normalized Expressions or Mean Normalized Expressions. In this contribution it will be shown that the calculation of Mean Normalized Expressions has ...
The real-time reverse transcription polymerase chain reaction (RT-PCR) uses fluorescent reporter molecules to monitor the production of amplification products during each cycle of the PCR reaction. This combines the nucleic acid amplification and detection steps into one homogeneous assay and obviates the need for gel electrophoresis to detect amplification products. Use of appropriate chemistr...
objective: in this study, a sybr green real-time rt-pcr assay for quantification of hiv-1 viral rna was developed. materials and methods: this assay was performed based on amplification of the pol region of hiv-1 and product analysis by an abi 7500 system. we quantified hiv-1 viral load in 26 seropositive patients by this system and the data were subsequently compared with results obtained wit...
the polyphenol silybin has anti-oxidant and anti-cancer properties. the poor bioavailability of some polyphenols (flavonoids, and terpenoids) can be improved by binding them to phosphatidylcholine (phytosome technology). many studies have focused on the most common phytosome, silybin-phosphatidylcholine, particularly for its hepatoprotective effects. however, in recent years, studies have also ...
زمینه و هدف: در سال های اخیر با افزایش اپیدمی های ویروس های آنفلوانزای a تحقیقات زیادی در مورد پیشگیری و درمان این ویروس ها انجام شده است. داروی اسلتامیویر یا تامی فلو (مهارکننده نورامینیداز (na) ویروس) به عنوان یکی از داروهای مؤثر در پیشگیری و درمان این ویروس ها می باشد. جهش در چند سایت مختلف ژن na باعث ایجاد مقاومت دارویی می گردد. جهش h274y از مهم ترین تغییرات ایجادکننده مقاومت دارویی در ویرو...
بررسی بیان ژن her 2 در بافت سرطانی پستان سگ به روش real time pcr چکیده: هدف اصلی این تحقیق بهینه سازی روش relative quantitative real-time pcr مبتنی بر سایبر گرین جهت اندازه گیری نسبی بیان ژن her2 در نمونه های مبتلا به سرطان پستان و طبیعی در سگ بود.8 نمونه سالم و 8 نمونه سرطانی پستان سگ قبل از درمان و بدون آلودگی جمع آوری شدند. rnaی کل بافت های پستانی ، استخراج و قطعات ژنی her2 و ? -actin توسط...
Rapid Detection of COVID-19 by RT-LAMP PCR Technique and its Comparison with Real-Time RT-PCR Method
Rapid antigen and antibody, serological tests, and RT ‑ PCR-based molecular methods are widely used for the detection of microorganisms worldwide. Objectives: This study aimed by Isothermal nucleic acid amplification techniques to detect the covid-19. Methods: In this study, 200 samples of nasopharynx and oropharynx were collected from Jamaran Heart Hospital in Tehran. Covid -19 was exami...
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