UNLABELLED The transcription factors HilA and SsrB activate expression of two type III secretion systems (T3SSs) and cognate effectors that reprogram host cell functions to benefit infecting Salmonella in the host. These transcription factors, the secretion systems, and the effectors are all encoded by horizontally acquired genes. Using quantitative proteomics, we quantified the abundance of 2,...

Salmonella enterica invades the intestinal epithelium of the host using a type III secretion system encoded on Salmonella pathogenicity island 1 (SPI1). The bacteria integrate environmental signals from a variety of global regulatory systems to precisely induce transcription of SPI1. The regulatory circuit converges on expression of HilA, which directly regulates transcription of the SPI1 appar...

Modern web applications often contain features, such as landmarks, access control, or adaptation, that are difficult to model modularly with existing Model-Driven Web Engineering approaches. We show how HILA, an aspectoriented extensions for UML state machines, can represent these kinds of features as aspects. The resulting models achieve separation of concerns and satisfy the “Don’t Repeat You...

HilA activates the transcription of genes on Salmonella pathogenicity island 1 (SPI1), which encodes a type III secretion system (TTSS). Previous studies showed that transposon insertions in orgC, a gene located on SPI1, increase hilA expression. We characterize the orgC gene product and show that it is secreted via the SPI1 TTSS. We propose a model whereby OrgC functions as a secreted represso...

Mutual exclusion is a very common requirement in parallel systems. Yet its modeling is a tedious task in UML state machines, one of the most popular languages for behavior modeling. We present HiLA, an aspect-oriented extension of UML state machines, to address this problem. In HiLA, mutual exclusion can be modeled in a highly modular and declarative way. That is, the logic of mutual exclusion ...

Salmonella enterica serovar Typhimurium harbors five pathogenicity islands (SPI) required for infection in vertebrate hosts. Although the role of SPI1 in promoting epithelial invasion and proinflammatory cell death has been amply documented, SPI4 has only more recently been implicated in Salmonella virulence. SPI4 is a 24-kb pathogenicity island containing six open reading frames, siiA to siiF....

It is proposed that the dematiaceous hyphomycete Cladosporium bantianum (Saccardo) Borelli be transferred to the genus Xylohypha (Fries) Mason as Xylohypha bantiana (Saccardo) McGinnis, Padhye, Borelli, et Ajello. This new combination is necessary because X. bantiana produces conidiophores that are indistinguishable from its vegetative hyphae and one-celled, smooth-walled conidia that are borne...

Leucine-responsive regulatory protein (Lrp) is a global gene regulator that influences expression of a large number of genes including virulence-related genes in Escherichia coli and Salmonella. No systematic studies examining the regulation of virulence genes by Lrp have been reported in Salmonella. We report here that constitutive expression of Lrp [lrp(Con)] dramatically attenuates Salmonell...

Salmonella enterica serovar Typhimurium encounters numerous host environments and defense mechanisms during the infection process. The bacterium responds by tightly regulating the expression of virulence genes. We identified two regulatory proteins, termed RtsA and RtsB, which are encoded in an operon located on an island integrated at tRNA(PheU) in S. enterica serovar Typhimurium. RtsA belongs...