Despite the broad use of enzymes in electroanalytical biosensors, the influence of enzyme kinetics on the function of prototype sensors is often overlooked or neglected. In the present study, we employ amperometry as an alternative or complementary method to study the kinetics of tyrosinase, whose catalytic activity results in o-quinone products. We further compare our results for four monophen...

Glucose and cholesterol are biomolecules of prime importance to human life as they are associated with a number of diseases such as diabetes, cardiac diseases, kidney failure, etc. Quantitative determination of these biomolecules is very important in biochemistry, clinical chemistry and food biology. Numerous methods such as spectrometry, amperometry, HPLC, polarometry, and capillary electropho...

We examined the relative merits of direct-current, pulse, and differential pulse amperometry at a flow-through, glassy-carbon electrode used in monitoring column chromatography. We give a rapid, simple procedure for determining theophylline (1,3-dimethylxanthine) in 20--100 microliter of blood serum. Interference by 1,7-dimethylxanthine, a metabolite of caffeine, is compared for photometric and...