Whole blood has been found to be a reliable matrix for the detection and quantitation of cytomegalovirus (CMV) DNA. In this study, the performance of the artus CMV LightCycler (LC) PCR kit in conjunction with automated sample preparation on a BioRobot EZ1 workstation was evaluated. The accuracy, linearity, analytical sensitivity, and inter- and intra-assay variations were determined. A total of...

Sample preparation and DNA extraction protocols for DNA amplification by PCR, which can be applied in human fecal samples for taeniasis diagnosis, are described. DNA extracted from fecal specimens with phenol/chloroform/isoamilic alcohol and DNAzol reagent had to be first purified to generate fragments of 170 pb and 600 pb by HDP2-PCR. This purification step was not necessary with the use of QI...

Automation of the PacBio 10 kb Template Preparation using the SMRTbell Template Prep kit for the sequencing of bacterial genomes on the Agilent Automated Liquid Handling Platform is described. Four bacterial genomes of differing GC content are used to prepare libraries without bias reading for next generation sequencing. The gDNA quality assessment was done using spectrophotometric and automate...

The Agilent DNA Extraction Kit (p/n 200600) was compared to standard methods such as beadbeating and enzyme treatment for preparation of genomic DNA from the prokaryote Listeria monocytogenes. Using this extraction kit, with modifications, to lyse the bacteria and isolate high molecular weight DNA reproducibly yielded high quality DNA suitable for further applications such as polymerase chain r...

this report describes the histological and immunohistochemical features of a small intestinal tumor that resembled a human gastrointestinal stromal tumor (gists) in a seven-year-old male cross-breed dog. this was unique because of the expression of desmin by the tumor. grossly, the white-gray tumor measured 8.0 × 4.0 × 6.5 cm and was 760 gram in weight. it was cystic and enveloped the jejunum. ...

Levels of cortisol in fish blood provide quantitative information on the degree of stress induced by a variety of stressors. It is also useful in describing the social status of individual fish within groups. The commercial production of radioimmunoassay (RIA) kits, such as the DPC Coat-A-Count radioimmunoassay kit, has considerably reduced the effort required for cortisol measurement. These ki...

introduction: 99mtc-trodat-1 is a promising new radiopharmaceutical with the potential for routine use as the radiopharmaceutical for dopamine transporters scintigraphy as far as its image quality and daily availability are concerned. here we describe the development of a freeze-dried kit formulation based on the tricine exchange labeling approach for the preparation of this radiopharmaceutical...

Radiolabeled Microspheres of human serum albumin (MSA) is of great importance in the analysis of regional lung perfusion. Preparation of MSA kit has been installed in our Division recently is carried out in two stages: 1. Production of albumin sphere particles with suitable sizes by using the spinning disk technique. 2. Formulation of MSA as a radiopharmacutical kit. So it can be labelled...