alstroemeria (alstroemeriaceae family) is an ornamental monocot mainly produced for its cut flowers. embryogenic calluses have been employed for gene transformation in alstroemeria. this research work was undertaken to study the embryogenic callus induction in alstroemeria cv. fuego. the vegetative explants (node, internode, leaf) along with various concentrations of auxins (picloram, naa, iaa,...

Background: Chrysanthemum; also commonly known as mums or chrysanths, is one of the most important ornamental crops worldwide. Introducing desirable traits into this valuable plant by the conventional breeding has so far been faced with some restrictions due to the limited gene pool and cross-incompatibility. Therefore, breeders have decided to exploit Agrobacterium-mediated tr...

Effects of application in vivo of glyphosate, fluridone, and paclobutrazol to glasshouse-grown donor plants of Pennisetum purpureum Schum. on endogenous levels of abscisic acid (ABA) and indole-3-acetic acid (IAA) in young leaves and on somatic embryogenesis in cultured leaf explants were studied. Treatment of plants with glyphosate (100 milligrams per liter) resulted in elevated levels of endo...

Thymus daenensisis a perennial plant belonging to Lamiaceae family that is a species endemic to Iran. Due to the indiscriminate use of this plant which possesses medicinal values, T. daenensis tissue culture is suggested to increase the quality and quantity of its effective compounds. Callus cultures using leaf and stem explants were subjected to different hormonal treatments. Ten-millimeter ex...

The effect of exogenous auxin and cytokinin on cell proliferation and differentiation varies with the tissue system, and depends on the endogenous levels of the two hormones in the tissue. In this report, cytokinin (N-benzyladenine, BA) and auxin (indole-3-acetic acid, IAA) were used to treat young seedlings, leaf explants, and genetic tumors of a Nicotiana hybrid. Exogenous BA markedly acceler...

Rhizome explants were used to develop a protocol for in vitro plantlet regeneration of Podophyllum hexandrum, a critically endangered medicinal plant, through direct organogenesis. Highest rate of multiple shoot formation was noted in MS medium supplemented with 11.42 μM IAA within three months. A synergistic effect of 2.68 μM NAA and 11.1 μM BAP was second best. The 1⁄2 MS liquid medium with 1...

Sequences homologous to a potato cathepsin D inhibitor cDNA, p749, were identified in the genomic DNA of tomato (Lycopersicon esculentum) and of two non-tuber-bearing potato species (Solanum etuberosum and S. brevidens) by means of Southern blot analysis. The expression of these p749 genes in leaves was induced at the RNA level in response to wounding. High levels of p749 transcripts were detec...

This experiment was performed to optimize the medium for tissue culture of Taraxacum kok-saghyz Rodin. Different tissue culture approaches such as shoot regeneration from seed, callus formation from leaf explants and plant regeneration from callus were investigated in this study. All the explants were cultured on MS basal medium supplemented with 20g/l sucrose, 7g/l agar and different plant gro...

A protocol for direct organogenesis in Bixa orellana (pink flowers variety) has been developed with significant organogenic response from rooted hypocotyls, hypocotyl segments, and cotyledonary leaf explants on Murashige and Skoog (MS) medium supplemented with 2.0 mg L thidiazuron and 0.25% coconut water or 7.0 mg L N-Benzyladenine and 0.1 mg L a-naphthalene acetic acid. Thidiazuron in combinat...