Among enzymes, lipases have been widely investigated because of the numerous industrial applications. In this study, optimization of lipase production by Aspergillus niger in solid state fermentation from rice bran as solid substrate was investigated. The optimal conditions with the aid of central composite design (CCD) under response surface methodology (RSM) were obtained.  In the analysis of...

The tremendous mounting interest in lipase production is pertinent to the biotechnological applications that these enzymes offer. Lipase, belonging to the hydrolyze enzymes, is involved in copious biological functions including: hydrolysis insoluble triacylglycerol to mono and diacylglycerols and glycerol. Submerge fermentation technique was applied for the efficient production of this. Briefly...

lipases are diversified enzymes in their properties and substrate specificity, which make them attractive tools for various industrial applications. in this study, an alkaline thermostable lipase producing bacteria were isolated from soil of different regions of isfahan province (iran) and its lipase was purified by ammonium sulfate precipitation and ion exchange chromatography. to select a the...

Background: Lipase is an enzyme with immense application potential. Ester synthesis by lipase catalysis in organic media is an area of key industrial relevance. Enzymatic preparations with traits that cater to the needs of this function are hence being intensely researched. Objective: The objectives of the study were to immobilize the lipase from Bacillus sp. PS35 by cross-linking and adsorpti...

The present study was undertaken to screen the soil samples collected in Iran for the presence of the Bacillus subtilis lipase A gene. The bacterial colonies obtained from the collected soil samples were examined by physical appearance, biochemical tests and  the polymerase chain reaction (PCR). Only four colonies were identified as putative B. subtilis strains and all contained the lipase A ge...

Lipoprotein lipase activity in adipose tissue responds rapidly to changes in the physiological state. To study what mechanisms are involved in the regulation, guinea pigs were fasted and the decrease in adipose-tissue lipoprotein lipase activity was compared with the decreases in mRNA and lipase synthesis. The mRNA pattern (three species) did not change. There was a close parallelism between th...

Pseudomonas aeruginosa EF2, ATCC 9027 and ATCC 19660 were grown in a continuous culture under Tween 80 (polyoxyethylene sorbitan monooleate) limitation and optimum conditions (pH 6.5, 37°C at dilution rate of 0.05/h). Culture supernatants were carefully removed and stored at -20°C. To purify the lipases, the culture supernatant was reduced in volume to approximately 10 mL by an ultrafiltra...

Immunoprecipitations of hepatic lipase from pulse-labeled rat liver have demonstrated that hepatic lipase is synthesized in two distinct molecular weight forms, HL-I (Mr = 51,000) and HL-II (Mr = 53,000). Both forms are immunologically related to purified hepatic lipase, but not to lipoprotein lipase. HL-I and HL-II are also kinetically related and represent different stages of intracellular pr...

Lipase plays an important role in industrial and biotechnological applications. Lipases have been subject to modification at the N and C terminals, allowing better understanding of lipase stability and the discovery of novel properties. A thermotolerant lipase has been isolated from Antarctic Pseudomonas sp. The purified Antarctic AMS3 lipase (native) was found to be stable across a broad range...