Background: Cryopreservation of ovaries is an important technique in assisted reproduction technology. Physical forces like hydrostatic pressure have a pivotal role in reproduction systems. Due to changes in intrafollicular pressure during ovulatory process, this study designed to examine the effects of hydrostatic pressure on oocyte maturation and cell death in cumulus cells from cryopreserved...

Background: It is important to protect oocytes and embryos from oxidative stress in the culture medium. Melatonin has been shown to be a direct free radical scavenger. Therefore, effect of melatonin during in vitro oocyte maturation (IVM), fertilization and embryo development of mouse oocytes was evaluated. Materials and Methods: Oocytes from supper-ovulated mouse were divided to two groups: cu...

background: the aim of the current study was to assess the mrna levels of two mitochondria-related genes, including nuclear-encoded nrf1 (nuclear respiratory factor 1), mitochondrial transcription factor a (tfam), and mitochondrial-encoded cytochrome c oxidase subunit 1 (mt-co1) genes in various stages of the human oocyte maturation. methods: oocytes were obtained from nine infertile women with...

cyclophosphamide (cp) is one of the anti-neoplastic drugs. despite its numerous clinical applications, it has devastating effects on the testicles and declines the sperm quality in treated patients. this study was aimed to investigate the protective effect of crocin in improving the toxicity induced by cp in reproductive system. in this study, 24 male adult mice (6 to 8 weeks) were randomly div...

To evaluate the effect of follicular fluid on in vitro maturation, pig oocytes were cultured in the presence of hormones where 10% of fetal calf serum (FCS), 10% of follicular fluid from large follicles (l-pFF), 10% of follicular fluid from medium follicles (m-pFF) or no supplement were added. When oocytes where matured in medium containing the hormones the addition of different supplements did...

Background: The objectives of this study were: a) to observe the influence of cumulus investment expansion on the nuclear chromatin configuration of canine oocytes matured in vitro; b) to examine the relationship between cumulus cell (CC) expansion and its morphology after in vitro maturation (IVM); c) to ascertain the influence of in vivo serum progesterone (SP) concentrations of ovary donors ...

Background: The aim of the current study was to assess the mRNA levels of two mitochondria-related genes, including nuclear-encoded NRF1 (nuclear respiratory factor 1), mitochondrial transcription factor A (TFAM), and mitochondrial-encoded cytochrome c oxidase subunit 1 (MT-CO1) genes in various stages of the human oocyte maturation. Methods: Oocytes were obtained from nine infertile women wit...

Background Oocyte maturity includes nuclear and cytoplasmic maturity, both of which are important for embryo fertilization. The development of oocyte is not limited to the period of follicular growth, and starts from the embryonic period and continues throughout life. In this study, for the purpose of evaluating the effect of the FSH hormone on the expression of genes, GEO access codes for this...