in order to study the periplasmic expression of human growth hormone (hgh) in escherichia coli, the related cdna was inserted in two expression plasmids carrying pelb signal peptide, one with lac bacterial promoter and the other with a bacteriophage t7-based promoter. the recombinant plasmids were moved to tg1 and bl21 strains of e. coli, respectively. to induce the expression systems, iptg and...

reteplase is a segment of tissue plasminogen activator used for the removal of thrombi in blood vessels. in the present study the cloned reteplase gene was used for its expression in competent e. coli. the  recombinant plasmid, pet15b/reteplase (rpet-bl21), was transformed into competent e.coli strain bl21 (de3) cells. overnight culture of the transformed bacteria was induced by the addition of...

conclusions it can be concluded that the rsm is an effective method for the optimization of recombinant protein expression using synthetic genes in e. coli. results the proposed optimum fermentation condition consisted of 7.81 g l-1 glucose, od 600 nm prior induction 1.66 and induction temperature of 30.27°c. the model prediction of 0.267 g l-1 of rhifn-β and 0.961 g l-1 of acetate at the optim...

reteplase is a potent thrombolytic agent which is widely used in the management of acute myocardial infarction and stroke. it belongs to the third generation of the thrombolytic drugs and has been derived from native human tissue plasminogen activator by removing three domains of it and keeping the kringle 2 and serine protease domains. however, the high cost of this drug, has limited the appli...

the central role of dendritic cells (dcs) as bridging innate and adaptive immunity leads to the expanding use of these cells in the poultry vaccine studies. the most effective way to produce enough dcs is monocyte transformation by combined induction of interleukin-4 (il-4) and granulocyte-macrophage colony-stimulating factor (gm-csf). in this study full length of chicken il-4 (cil-4) cdna was ...

in recent year, the evolutionary structural optimization (eso) method, has been applied as an effective tool for structures design. the concept of eso method is to use the finite element  results in each iteration of process and removal of inefficient material based on these results. in this method there are several experimental parameters such as rejection rate and evolutionary rate that are e...